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Similarities of adenosine uptake systems in astrocytes and neurons in primary cultures

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Abstract

Uptake of extracellular adenosine was studied in primary cultures of astrocytes or neurons. Both cell types showed a high affinity uptake. TheK m values were not significantly different (6.5±3.75 μM in astrocytes and 6.1±1.86 μM in neurons), but the intensity of the uptake was higher in astrocytes than in neurons (V max values of 0.16±0.030 and 0.105±0.010 nmol×min−1×mg−1 protein, respectively). The temperature sensitivity was similar in the two cell types. Adenosine uptake inhibitors and benzodiazepines inhibited the adenosine uptake systems in both astrocytes and neurons with IC50 values in the high nanomolar or the micromolar range and the rank order of potency was similar in the two cell types. In both cell types the (−) isomers of two sets of benzodiazepine stereoisomers were more potent than the (+) isomers. Dixon analysis showed that dipyridamole, papaverine, hexobendine and chlordiazepoxide inhibited the adenosine uptake competitively and clonazepam noncompetitively in both cell types.

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  1. Department of Pharmacology, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada

    Alexander S. Bender & Leif Hertz

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Bender, A.S., Hertz, L. Similarities of adenosine uptake systems in astrocytes and neurons in primary cultures. Neurochem Res 11, 1507–1524 (1986). https://doi.org/10.1007/BF00965770

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