Abstract
An enzyme that catalyzes the conversion of 2-amino-6(-5′-triphosphoribosyl)amino-5- or 6-formamido-6-hydroxypyrimidine, but not of guanosine triphosphate, to quinonoid 6-(d-erythro-1′-2′-3′-trihydroxypropyl)dihydropterin triphosphate and formic acid has been purified to homogeneity from some mammalian brain and liver. The enzyme of a single strand is a basic protein of 9177 daltons consisting of 68 amino acid residues—except the enzyme from rat brain, which has one additional aspartic acid as residue 7. The enzyme possesses three free SH groups and, in its most active form, 1 mol of phosphate per mole of enzyme. Peptides isolated after hydrolysis with trypsin, chymotrypsin, or weak acid were separated by thin-layer chromatography and sequenced manually by Edman degradation. The complete sequence of the molecule was established as follows:
Similar content being viewed by others
References
Yim, J. J., andBrown, G. M. 1976. Characteristics of guanosine triphosphate cyclohydrolase I purified fromEscherichia coli, J. Biol. Chem. 251:5087–5094.
Fukushima, K., Richter, W. E., Jr., andShiota, T. 1977. Partial purification of 6-(d-erythro-1′-2′-3′-trihydroxypropyl)-7,8-dihydropterin triphosphate synthetase from chicken liver.J. Biol Chem. 252:5750–5755.
Gál, E. M., Nelson, J. M., andSherman, A. D. 1978. Biopterin. III. Purification and characterization of enzymes involved in the cerebral synthesis of 7,8-dihydrobiopterin.Neurochem. Res. 3:69–88.
Gál, E. M., Bybee, J. A., andSherman, A. D. 1979. Biopterin. V.De novo synthesis of dihydrobiopterin: Evidence for its quinonoid structure and lack of dependence of its reduction to tetrahydrobiopterin on dihydrofolate reductase.J. Neurochem. 32:179–186.
Winter, A., Perlmutter, H., andDavies, H. Preparative flat-bed electrofocusing in a granulated gel with the LKB 2117 Multiphor.LKB Application Bull. No. 198.
Waddell, W. J. 1956. A simple ultraviolet spectrophotometric method for the determination of protein.J. Lab. Clin. Med. 48:311–314.
Slobin, L. I. 1971. Sulfhydryl groups ofEscherichia coli ribosomes and ribosomal proteins.J. Mol. Biol. 61:281–285.
Mende, T. J., andWhitney, P. L. 1978. Microdetermination of inorganic sulfate using thin-layer plates.Anal. Biochem. 84:570–573.
Gál, E. M., andSherman, A. D. 1978. Phosphorylation, a factor controlling the synthesis ofl-erythrodihydrobiopterin (BH2).Biochem. Biophys. Res. Commun. 83:593–598.
Moss, C. W., Lambert, M. A., andDias, F. J. 1971. Gas-liquid chromatography of twenty protein amino acids on a single column.J. Chromatog. 60:134–136.
Gehrke, C. W., Roach, D., Zumwalt, R. W., Stalling, D. L., andWall, L. L. 1968.Quantitative Gas-Liquid Chromatography of Amino Acids in Proteins and Biological Samples. Anal. Biochem. Labs., Columbia, Mo.
Smyth, D. G. 1967. Techniques in enzymic hydrolysis. Pages 214–231,in Hirs, C. H. W. (ed.),Methods in Enzymology, Vol. XI. Academic Press, London.
Light, A. 1967. Partial acid hydrolysis. Pages 417–420,in Hirs, C. H. W. (ed.),Methods in Enzymology, Vol. XI. Academic Press, London.
Ritschard, W. J. 1964. Thin-layer techniques for making peptide maps.J. Chromatog. 16:327–334.
Riordan, J. F., Wacker, W. E. C., andVallee, B. L. 1965.N-Acetylimidazole: A reagent for determination of “free” tyrosyl residues of proteins.Biochemistry 4:1758–1765.
Bruton, C. J., andHartley, B. S. 1970. Chemical studies on methionyl-tRNA synthetase fromEscherichia coli.J. Mol. Biol. 52:165–178.
Lamkin, W. M., Weatherford, J. W., Jones, N. S., Pan, T., andWard, D. N. 1974. Analysis of methylthiohydantoins of amino acids by gas-liquid chromatography of their trimethylsilyl derivatives.Anal. Biochem. 58:422–438.
Ouchterlony, O. 1968.Handbook of Immunodiffusion and Immunoelectrophoresis. Ann Arbor Humphrey Science Publishers, Ann Arbor, Mich.
Brown, G. M., andFan, C. L. 1975. The synthesis of pterins catalyzed by enzymes fromDrosophila melanogaster. Pages 265–271,in Pfleiderer, W. (ed.),Chemistry and Biology of Pteridines. Walter de Gruyter, New York.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gál, E.M., Dawson, M.R., Dudley, D.T. et al. Biopterin. Neurochem Res 4, 605–626 (1979). https://doi.org/10.1007/BF00964438
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00964438