Summary
Maximal molar growth yields (Y maxsub ) and protease production ofBacillus licheniformis S 1684 during NH +4 -, O2-, and NH +4 +O2-limitation with either glucose or citrate as carbon and energy source and during glucose-, and citratelimitation in chemostat cultures were determined. Protease production was repressed by excess ammonia when glucose served as C/E-source. Glucose and citrate repressed protease production during NH +4 -limitation. A low oxygen tension enbanced protease production at low μ-values. It was concluded that, besides ammonia repression, catabolite flux and oxygen tension influence protease production, indicating that the energy status of the cell is important for the level of protease production.Y maxsub -values were high during glucose-limitation and indicate a high efficiency of growth caused by a highY maxATP . During NH +4 -, O2-, and NH +4 +O2-limitation with glucose as C/E-values were lower than during glucose limitation. The lowerY maxsub -values were due to a lower efficiency of energy conservation.Y maxsub -values during limitations with citrate as C/E-source were lower than during limitations with glucose as C/E-source.
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Abbreviations
- μ:
-
specific growth rate (h-1)
- Y sub :
-
growth yield per mol substrate (g biomass/mol)
- Y max :
-
maximal molar growth yield corrected for maintenance requirements (g biomass/mol)
- Y max (corr):
-
Y max corrected for product formation (g biomass/mol)
- m sub :
-
maintenance requirements (mol/g biomass·h)
- m sub (corr):
-
maintenance requirements corrected for product formation (mol/g biomass·h)
- q maxport :
-
maximal specific rate of protease production (E440/mg DW·h)
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Frankena, J., Koningstein, G.M., van Verseveld, H.W. et al. Effect of different limitations in chemostat cultures on growth and production of exocellular protease byBacillus licheniformis . Appl Microbiol Biotechnol 24, 106–112 (1986). https://doi.org/10.1007/BF00938779
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DOI: https://doi.org/10.1007/BF00938779