References
Allsopp BA, Allsopp MTEP (1988)Theileria parva genomic DNA studies reveal intra-species sequence diversity. Mol Biochem Parasitol 28:77–84
Allsopp BA, Gibson WC, Stagg DA (1985) Characterization of some East AfricanTheileria species isolates by isoenzyme analysis, with particular reference toT. parva. Int J Parasitol 15:271–276
Anderson NG, Anderson NL (1978a) Analytical techniques for cell fractions: XXI. Two-dimensional analysis of serum and tissue proteins: multiple isoelectric focusing. Anal Biochem 85:331–340
Anderson NL, Anderson NG (1978b) Analytical techniques for cell fractions: XXII. Two-dimensional analysis of serum and tissue proteins: multiple gradient slab gel electrophoresis. Anal Biochem 85:331–340
Conrad PA, Iams K, Brown WC, Sohanpal B, ole-MoYoi OK (1987a) DNA probes detect genomic diversity inTheileria parva stocks. Mol Biochem Parasitol 25:213–226
Conrad PA, Stagg DA, Grootenhuis JG, Irvin AD, Newson J, Najamunggeh REG, Rossiter PB, Young AS (1987b) Isolation ofTheileria parasites from African buffalo (Syncerus caffer) and characterization with anti-schizont monoclonal antibodies Parasitology 94:413–423
Conrad PA, ole-MoiYoi OK, Baldwin CL, Dolan TT, O'Callaghan CJ, Njamunggeh REG, Grootenhuis JG, Staag DA, Leitch BL, Young AS (1989a) Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes. Parasitology 98:179–188
Conrad PA, Baldwin CL, Brown WC, Sohanpal B, Dolan TT, Goddeeris BM, Demartini JC, ole-MoiYoi OK (1989b) Infection of bovine T cell clones with genotypically distinctTheileria parva parasites and analysis of their cell surface phenotype. Parasitology 99:205–213
Dunn SD (1986) Effects of the modification of transfer buffer composition and renaturation of proteins in gels on the recognition of proteins of Western blots by monoclonal antibodies. Anal Biochem 157:144–153
Goddeeris BM, Katende JM, Irvin AD, Chumo RSC (1982) Indirect immunofluorescent antibody test for experimental and epizootiological studies on East Coast fever (Theileria parva infection in cattle). Evaluation of a cell culture schizont antigen fixed and stored in suspension. Res Vet Sci 33:360–365
Irvin AD (1987) Characterization of species and strains ofTheileria. Adv Parasitol 26:145–197
Irvin AD, Dobbelaere DAE, Mwamachi DM, Minami T, Spooner PR, Ocama JGR (1983) Immunization against East Coast fever: correlation between monoclonal antibody profiles ofTheileria parva stocks and cross-immunity in vivo. Res Vet Sci 35:341–346
Koch HT, Ocama JGR, Munatswa FC, Byrom B, Norval RAI, Spooner PR, Conrad PA, Irvin AD (1988) Isolation and characterization of bovineTheileria parasites in Zimbabwe. Vet Parasitol 28:19–32
Melrose TR, Brown CGD, Sharma RD (1980) Glucose phosphate isomerase isoenzyme patterns in bovine lymphoblastoid cell lines infected withTheileria annulata andT. parva. Res Vet Sci 29:298–304
Melrose TR, Brown CGD, Morzaria SP, Ocama JGR, Irvin AD (1984) Glucose phosphate isomerase polymorphism inTheileria annulata andT. parva. Trop Anim Health Prod 16:239–245
Minami T, Spooner PR, Irvin AD, Ocama JGR, Dobbelaere DAE (1983) Characterization of stocks ofTheileria parva by monoclonal antibody profile. Res Vet Sci 35:334–340
Pinder M, Hewett R (1980) Monoclonal antibodies detect antigenic diversity inTheileria parva parasites J Immunol 124:1000–1001
Radley DE (1981) Infection and treatment method of immunization against theileriosis. In: Irvin AD, Cunningham MP, Young AS (eds) Advances in the control of theileriosis. Martinus Nijhoff, The Hague, pp 227–237
Shapiro SZ, Fujisaki K, Morzaria SP, Webster P, Fujinaga T, Spooner PR, Irvin AD (1987) A life cycle stage-specific antigen ofTheileria parva recognized by anti-schizont monoclonal antibodies. Parasitology 94:29–37
Sugimoto C, Conrad PA, Ito S, Brown WC, Grab DJ (1988) Isolation ofTheileria parva schizont from infected lymphoblastoid cells. Acta Trop (Basel) 45:203–216
Sugimoto C, Conrad PA, Mutharia L, Dolan, TT, Brown WC, Goddeeris BM, Pearson TW (1989) Phenotypic characterization ofTheileria parva schizonts by two-dimensional gel electrophoresis. Parasitol Res 76:1–7
Uilenberg G (1981) Theilerial species of domestic livestock. In: Irvin AD, Cunningham MP, Young AS (eds) Advances in the control of theileriosis. Martinus Nijhoff, The Hague, pp 4–37
Author information
Authors and Affiliations
Additional information
ILRAD publication 943
Rights and permissions
About this article
Cite this article
Sugimoto, C., Mutharia, L.M., Brown, W.C. et al. Analysis ofTheileria parva immunodominant schizont surface antigen by two-dimensional polyacrylamide gel electrophoresis and immunoblotting. Parasitol Res 78, 82–85 (1992). https://doi.org/10.1007/BF00936188
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00936188