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Vitreal and retinal oxygenation

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Abstract

This paper reports the results of experiments carried out to understand the oxygenation of the normal retina in response to alterations in physiological conditions such as inspired oxygen concentration, elevated IOP, luminance changes and occlusion of the retinal circulation. Measurements of vitreal and intraretinal PO2 in vivo in the cat using oxygen-sensitive microelectrodes demonstrated that large PO2 gradients were set up preretinally and that arterial, venous and tissue PO2 increased when inspired PO2 was raised to 100% O2. Results showed that with an occluded retinal circulation, it was possible to oxygenate fully the whole retina with oxygen supplied from a hyperoxic choroidal circulation. With alterations in background luminance from photopic to scotopic, preretinal PO2 was unaffected for air breathing, whereas for 100% O2, breathing vitreal PO2 fell quickly and reversibly on a switch from photopic to scotopic conditions, reflecting an increase in retinal oxygen consumption in a dark environment. During acute rises of IOP, the PO2 at the choriocapillaris fell and an anoxic region developed in the middle retinal layers. The inner retina was relatively resistant to rises in IOP. The implications of these data for autoregulation of the retinal circulation are discussed.

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References

  1. Alder VA, Cringle SJ (1985) The effect of the retinal circulation on vitreal oxygen tension. Curr Eye Res 5:121–129

    Google Scholar 

  2. Alder VA, Cringle SJ (1989) Intraretinal and preretinal PO2 response to acutely raised intraocular pressure in cats. Am J Physiol 256:1627–1634

    Google Scholar 

  3. Alder VA, Cringle SJ, Constable IJ (1983) The retinal oxygen profile in cats. Invest Ophthalmol Vis Sci 24:30–36

    Google Scholar 

  4. Alm A, Bill A (1972) The oxygen supply to the retina: 1. Effects of changes in intraocular and arterial blood pressures, and arterial PO2 and PCO2 on the oxygen tension of the vitreous body of the cat. Acta Physiol Scand 84:261–274

    Google Scholar 

  5. Alm A, Bill A (1972) The oxygen supply to the retina: 2. Effects of high intraocular pressure and of increased arterial carbon dioxide tension on uveal and retinal blood flow in cats. Acta Physiol Scand 84:306–319

    Google Scholar 

  6. Ben-Nun J, Alder VA, Cringle SJ, Constable IJ (1988) A new method for oxygen supply to acute ischemic retina. Invest Ophthalmol Vis Sci 29:298–304

    Google Scholar 

  7. Brown KT (1968) The electroretinogram: its components and their origins. Vision Res 8:633–677

    Google Scholar 

  8. Dollery CT, Bulpitt CJ, Kohner EM (1969) Oxygen supply to the retina from the retinal and choroidal circulations at normal and increased arterial oxygen tensions. Invest Ophthalmol 8:588–594

    Google Scholar 

  9. Henkind P (1966) The retinal vascular system of the domestic cat. Exp Eye Res 5:10–15

    Google Scholar 

  10. Landers MB (1978) Retinal oxygenation via the choroidal circulation. Trans Am Ophthalmol Soc 76:528–556

    Google Scholar 

  11. Linsenmeier RA (1986) Effects of light and darkness on oxygen distribution and consumption in the cat retina. J Gen Physiol 88:521–542

    Google Scholar 

  12. Linsenmeier RA, Goldstick TK, Blum RS, Enroth-Cugell C (1981) Estimation of retinal oxygen tension transients from measurements made in the vitreous humor. Exp Eye Res 32:369–379

    Google Scholar 

  13. Molnar I, Poitry S, Tsacopoulos M, Giladi N, Leuenberger PM (1985) Effect of laser photocoagulation on oxygenation of the retina in miniature pigs. Invest Ophthalmol Vis Sci 26:1410–1414

    Google Scholar 

  14. Riva C, Grunwald J, Sinclair H (1983) Laser Doppler velocimetry study of the effect of pure oxygen breathing on retinal blood flow. Invest Ophthalmol Vis Sci 24:47–51

    Google Scholar 

  15. Riva C, Pournaras E, Tsacopoulos M (1986) Regulation of local oxygen tension and blood flow in the inner retina during hyperoxia. J Appl Physiol 61:592–598

    Google Scholar 

  16. Stefansson E, Wolbarsht M, Landers M (1983) In vivo oxygen consumption in rhesus monkeys in light and dark. Exp Eye Res 37:251–256

    Google Scholar 

  17. Tillis TN, Murray DL, Schmidt GJ, Welter JJ (1988) Preretinal oxygen changes in the rabbit under conditions of light and dark. Invest Ophthalmol Vis Sci 29:988–990

    Google Scholar 

  18. Tsacopoulos M, Baker R, Levy S (1976) Studies on retinal oxygenation. Adv Exp Med Biol 75:413–416

    Google Scholar 

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Authors and Affiliations

  1. Lions Eye Institute and Department of Surgery, University of Western Australia, 6009, Nedlands, Western Australia, Australia

    Valerie A. Alder & Stephen J. Cringle

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  1. Valerie A. Alder
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  2. Stephen J. Cringle
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Alder, V.A., Cringle, S.J. Vitreal and retinal oxygenation. Graefe's Arch Clin Exp Ophthalmol 228, 151–157 (1990). https://doi.org/10.1007/BF00935725

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  • DOI: https://doi.org/10.1007/BF00935725

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