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Induction of N-acetylglucosaminyltransferase V by elevated expression of activated or proto- Ha-ras oncogenes

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Abstract

Viral infection of cultured cells with transforming viruses causes an increase in cell-surface N-linked β1-6 (GlcNAcβ1-6Man) branching of complex-type oligosaccharides. Similar observations have been made after transfection of cells with activated oncogenes, which is associated with an induction of tumorigenic and metastatic properties. In this study, the effects of transfection of both activated and proto-Ha-ras oncogenes into NIH3T3 cells were analyzed. The results showed that, in comparison with NIH3T3 cells, bothras transfectants have increased sensitivity to the cytotoxic action of L-PHA. An increase in β1-6 branching and an increased level of N-acetylglucosaminyltransferase V (GlcNAc-T V), the enzyme which initiates the β1-6 branching were also observed. The levels of GlcNAc-T I and β1-4 Gal-T remained unchanged in activated Ha-ras transfected NIH3T3 cells. These data suggest that a specific induction of GlcNAc-T V occurs after transfection with either the proto- or activated Ha-ras oncogenes, which is responsible for the increased β1-6 branching previously observed. (Mol Cell Biochem122: 85–92, 1993)

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Abbreviations

Asn:

Asparagine

BHK:

Baby Hamster Kidney (cells)

β1-4 Gal-T or Gal-T:

β1-4 Galactosyltransferase

BSL-II:

Banderia Simplifolia Lectin II

CHO:

Chinese Hamster Ovary (cells)

ConA:

Concanavalin A

D-MEM:

Dulbecco's Modified Eagle's Medium

E-PHA:

Erythroagglutinin of Phytohemagglutinin

Fuc:

Fucose

Gn or GlcNAc:

N-acetylglucosamine

GlcNAc-T I:

N-acetylglucosaminyltransferase I

LCA:

Lens Culinaris Agglutinin

L-PHA:

Leukoagglutinin of Phytohemagglutinin

M or Man:

Mannose

PBS:

Phosphate-Buffered Saline

PL:

Pea Lectin

RIC:

Ricin

SDS-PAGE:

Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

UDP-Gal:

Uridine-Diphosphate-Galactose

UDP-Gn:

Uridine-Diphosphate-N-acetylglucosamine

WGA:

Wheat Germ Agglutinin

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 600 South 42nd Street, 68198-4525, Omaha, NE, USA

    Yi Lu & William Chaney

  2. The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 600 South 42nd Street, 68198-4525, Omaha, NE, USA

    William Chaney

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  1. Yi Lu
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Lu, Y., Chaney, W. Induction of N-acetylglucosaminyltransferase V by elevated expression of activated or proto- Ha-ras oncogenes. Mol Cell Biochem 122, 85–92 (1993). https://doi.org/10.1007/BF00925741

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  • DOI: https://doi.org/10.1007/BF00925741

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