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A lymphoproliferative disorder of the large granular lymphocytes with natural killer activity

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Abstract

This paper reports the case of a patient with an abnormally expanded population of circulating lymphoid cells displaying the features of the so-called large granular lymphocytes (LGL). These cells were peroxidase negative and nonphagocytic, formed rosettes with sheep erythrocytes, had receptors for IgG, and contained azurophilic (electron-dense) granules. Like normal LGL, the patient cells were positive for two acid hydrolases (acid phosphatase and β-glucuronidase) but did not stain for α-naphthyl acetate esterase (ANAE), which is present in normal LGL. Ultrastructural studies revealed that the patient cells were rich in Golgi-derived vesicles, coated vesicles, multivesicular bodies, and immature granules, indicating that, unlike normal LGL, they were engaged in granulogenesis. These features, together with the absence of ANAE activity, are suggestive of some degree of cell immaturity. The patient cells displayed natural killer (NK) and antibody-dependent cellular cytotoxicity (ADCC) activities comparable to those of normal peripheral blood mononuclear cells, or even higher, and did not respond to T-cell mitogens or allogeneic cells. Furthermore, they were incapable of suppressing normal T-cell proliferation or pokeweed mitogen-induced B-cell differentiation. Analysis of the NK activity at the single-cell level revealed that a large proportion of the patient cells bound to the K562 target cells but could not accomplish the entire lytic process. This finding supports further the possibility that the patient cells were immature LGL. The surface phenotype of the patient cells (as defined by a battery of monoclonal antibodies) was somewhat different from that usually observed in the majority of the normal LGL because, in addition to the HNK-1 marker, the cells were OKT3+, aLeul+, aLeu4+, OKT8+, aLeu2a+, and 3A1+ but were OKM1− and 4F2−. This phenotype could correspond to that of maturing LGL.

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Authors and Affiliations

  1. Departments of Immunology and Anatomy, University of Genova, 16131, Genova

    Manlio Ferrarini, Elisabetta Montesoro, Antonio Zicca, Arcangelo Nocera, Arnaldo Leprini & Carlo Enrico Grossi

  2. Department of Clinical Immunology, University of Firenze, Firenze, Italy

    Sergio Romagnani, Gian Franco del Prete & Enrico Maggi

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  1. Manlio Ferrarini
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  2. Sergio Romagnani
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  3. Elisabetta Montesoro
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Ferrarini, M., Romagnani, S., Montesoro, E. et al. A lymphoproliferative disorder of the large granular lymphocytes with natural killer activity. J Clin Immunol 3, 30–41 (1983). https://doi.org/10.1007/BF00919136

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