Abstract
We examined regulation of Epstein-Barr virus-induced plaque-forming cell generation in peripheral blood mononuclear cells from several autoimmune and seronegative diseases and correlated these results with Epstein-Barr virus-induced proliferation. We confirmed the defective regulation of Epstein-Barr virus-induced plaque-forming cells in peripheral blood mononuclear cells of patients with rheumatoid arthritis and scleroderma. Peripheral blood mononuclear cells from patients with seronegative arthropathies and chronic infective inflammation (cystic fibrosis) had normal regulation of Epstein-Barr virus-induced plaque-forming cells. Peripheral blood mononuclear cells from rheumatoid arthritis had excessive plaque-forming cell generation in the face of a normally regulated decrease in Epstein-Barr virus-induced proliferation. In contrast, peripheral blood mononuclear cells from scleroderma had defective suppression of both Epstein-Barr virus-induced proliferation and plaque-forming cell generation. Thus, impaired regulation of Epstein-Barr virus-induced plaque-forming cell generation is a common feature of autoimmune disease and demonstrates some specificity for these disorders.
Similar content being viewed by others
References
Tosato P, Magrath IT, Blaese M: T-cell mediated immunoregulation of Epstein Barr virus (EBV) B-lymphocyte activation in EBV-seropositive and EBV-seronegative individuals. J Immunol 128:575–579, 1982
Alspaugh MA, Jensen FC, Robin H, Tan EM: Lymphocytes transformed by Epstein-Barr virus: induction of nuclear antigen reactive with antibody in rheumatoid arthritis. J Exp Med 147:1018–1027, 1978
Alspaugh MA, Henle G, Lennetle ET, Henle W: Elevated levels of antibodies to Epstein-Barr virus antigens in sera and fluids of patients with rheumatoid arthritis. J Clin Invest 67:1134–1140, 1976
Depper JM, Bluestein HG, Zvaifler NJ: Impaired regulation of Epstein-Barr virus induced outgrowth in rheumatoid arthritis is due to a T-cell defect. J Immunol 127:1899–1902, 1981
Tosato G, Steinberg AD, Blaese RM: Defective EBV-specific suppressor T-cell function in rheumatoid arthritis. N Engl J Med 305:1238–1243, 1981
Tsokos GC, Magrath IT, Balow JE: Epstein-Barr virus induces normal B-cell responses but defective suppressor T-cell responses in patients with systemic lupus erythematosus. J Immunol 131:1797–1801, 1983
Kahan A, Kahan A, Menkes CJ, Amor B: Defective Epstein-Barr virus specific suppressor T-cell function in progressive systemic sclerosis. Ann Rheum Dis 45:553–560, 1986
Ropes MWG, Bennett GA, Cobb S,et al.: Revision of diagnostic criteria for rheumatoid arthritis. Arth Rheum 2:517–524, 1951
Masi AT, Rodnan GP, Medsger TA,et al.: Preliminary criteria for the classification of systemic sclerosis (scleroderma). Arth Rheum 23:581–590, 1980
Bennett PH, Burch TA: New York symposium on population studies in rheumatic diseases: New diagnostic criteria. Bull Rheum Dis 17:453–458, 1967
Tan PLJ, Shore A: Thymosin induces helper function in OKT3-positive, E-rosette negative human cord blood T-cells. Scand J Immunol 20:27–35, 1984
Burns GF, Pike BL: Spontaneous reverse haemolytic plaque formation. J Immunol Methods 41:269–277, 1981
Tan PLJ, Pang G, Wilson D: Immunoglobulin productionin vitro by peripheral blood lymphocytes in systemic lupus erythematosus: Helper T-cell defect and B-cell hyperreactivity. Clin Exp Immunol 44:548–554, 1981
Tan PLJ, Pang G, Cullinaue G, Wilson, D: Immunoglobulin-secreting cells in SLE: Correlation with disease activity. J Rheumatol 7:807–813, 1980
Bardwick PA, Bluestein HG, Zvaifler NJ,et al.: Altered regulation of Epstein-Barr virus induced lymphoblast proliferation in rheumatoid arthritis lymphoid cells. Arth Rheum 23:626–631, 1980
Stierle HE, Brown KA, Perry JD, Holborrow EJ: Increased responsiveness of rheumatoid B-lymphocytes to stimulation by Epstein-Barr virus. Rheumatol Int 3:7–11, 1983
Tan PLJ, Shore A: Two separate mechanisms for the feedback suppression of immunoglobulin production: Epstein-Barr virus induced B lymphoblastoid cell lines activate suppressor T-cells and enhance γ-interferon production. Arth Rheum 27:S56, 1984 (abstr)
Hasler F, Bluestein WG, Zvaifler NJ, Epstein LB: Analysis of the defects responsible for the impaired regulation of Epstein-Barr virus-induced B-cell proliferation by rheumatoid arthritis lymphocytes. J Exp Med 157:173–188, 1983
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shore, A., Klock, R., Lee, P. et al. Impaired late suppression of Epstein-Barr virus (EBV)-induced immunoglobulin synthesis: A common feature of autoimmune disease. J Clin Immunol 9, 103–110 (1989). https://doi.org/10.1007/BF00916937
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00916937