Abstract
A protease similar to papain was produced by somatic embryos ofCarica papaya in association with the development of laticifers containing characteristic vesicles which probably originated from the endoplasmic reticulum. In contrast to somatic embryos, a papain-like protease was not detected in either friable or compact callus cultures which failed to develop laticifers. These observations strongly suggest that the differentiation into laticifers is required for papain production in papaya.
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Amann M, Wanner G, Zenk MH (1986) Planta 167: 310–320
Bradford MM (1976) Anal. Biochem. 72: 248–254
Galewsky S, Nessler CL (1986) Plant Science 45: 215–222
Galneder E, Rueffer M, Wanner G, Tabata M, Zenk MH (1988) Plant Cell Rep. 7: 1–4
Hutabarat RM, Medora RS (1986) In: Abstracts of Proceedings of VI International Congress of Plant Tissue and Cell Culture (Sommer DA, Gengenbach BG, Biesboer DD, Hackett WB, Green CE eds.), IAPTC, University of Minnesota, pp. 364
Kutchan TM, Ayabe S, Krueger RJ, Coscia EM, Coscia CJ (1983) Plant cell Rep. 2: 281–284
Kutchan TM, Ayabe S, Coscia CJ (1985) In: The Chemistry and Biology of Isoquinoline Alkaloids (Phillipson JD, Roberts MF, Zenk MH eds.), Springer-Verlag, Berlin, Heidelberg, pp. 281–294
Kutchan TM, Rush M, Coscia CJ (1986) Plant Physiol. 81: 161–166
Linsmaier EM, Skoog F (1965) Physiol. Plant. 18: 100–127
Nessler CL, Mahlberg PG (1977) Amer. J. Bot. 64: 541–551
Roberts MF, McCarthy D, Kutchan TM, Coscia CJ (1983) Arch. Biochem. Biophys. 222: 599–609
Roberts MF (1987) In: Plant Vacuoles (Marin A ed.), Plenum Press, New York and London, pp. 513–528
Rush MD, Kutchan TM, Coscia CJ (1985) Plant Cell Rep. 4: 237–240
Tsukada M, Tabata M (1984) Planta Med. 51: 338–341
Yamamoto H, Tanaka S, Fukui H, Tabata M (1986a) Plant Cell Rep. 5: 269–272
Yamamoto H, Nakagawa N, Fukui H, Tabata M (1986b) Plant Cell Rep. 5: 65–68
Yamamoto H, Tabata M, Leistner E (1987) Plant Cell Rep. 6: 187–190
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Yamamoto, H., Tabata, M. Correlation of papain-like enzyme production with laticifer formation in somatic embryos of papaya. Plant Cell Reports 8, 251–254 (1989). https://doi.org/10.1007/BF00778545
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DOI: https://doi.org/10.1007/BF00778545