Abstract
To minimize artifacts in temperature-velocity (Arrhenius) profiles due to aging of preparations of biological membranes, a rapid controller of temperature was developed for spectrophotometric or polarographic (O2 electrode) measurements. The reaction mixture is cooled or heated through contact with Peltier elements. One Pt temperature sensor in the cuvette or electrode holder controls current flow into the Peltier units, and another Pt temperature sensor in the reaction mixture is used to read out the sample temperature on a meter or recorder, and to provide feedback control. The sample temperature can be reproducibly set to within 0.1°C, with a noise level of 0.04°C or less; a change of 4°C takes 1 min.
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References
Fox, C. F. (1975). InMTP International Review of Science: Biochemistry, Series 1 (Fox, C. F., ed.), Butterworths, London, pp. 279–306.
Hoch, F. L., DePierre, J. W., and Ernster, L. (1980).Eur. J. Biochem.,109 301–306.
Mela, L. T., and Chance, B. (1968).Biochemistry 11 4059–4063.
Neupert, M., Seifarth, H. H., Herrling, R., and Hahne, B. (1972).Jena Rev. 17 75–83.
Raison, J. K. (1973).J. Bioenerg. 4 285–309.
Shaw, M. J., and Hoch, F. L. (1977).J. Mol. Cell. Cardiol. 9 749–761.
Siemens AB, Avdelning Telekomponenter, Fack 104 35 Stockholm 23, Sweden: Peltier Cooling Block PKE 36 A 001.
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On leave at the Arrhenius Laboratory, University of Stockholm.
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Hoch, F.L., Höijer, B. A rapid controller of temperature for use in determining Arrhenius profiles in biomembrane systems. J Bioenerg Biomembr 13, 25–35 (1981). https://doi.org/10.1007/BF00744744
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DOI: https://doi.org/10.1007/BF00744744