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Monoclonal antibodies to the Ca2+ + Mg2+-dependent ATPase of sarcoplasmic reticulum identify polymorphic forms of the enzyme and indicate the presence in the enzyme of a classical high-affinity Ca2+ binding site

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Abstract

In order to determine whether polymorphic forms of the Ca2+ + Mg2+-dependent ATPase exist, we have examined the cross-reactivity of five monoclonal antibodies prepared against the rabbit skeletal muscle sarcoplasmic reticulum enzyme with proteins from microsomal fractions isolated from a variety of muscle and nonmuscle tissues. All of the monoclonal antibodies cross-reacted in immunoblots against rat skeletal muscle Ca2+ + Mg2+-dependent ATPase but they cross-reacted differentially with the enzyme from chicken skeletal muscle. No cross-reactivity was observed with the Ca2+ + Mg2+-dependent ATPase of lobster skeletal muscle. The pattern of antibody cross-reactivity with a 100,000 dalton protein from sarcoplasmic reticulum and microsomes isolated from various muscle and nonmuscle tissues of rabbit demonstrated the presence of common epitopes in multiple polymorphic forms of the Ca2+ + Mg2+-dependent ATPase. One of the monoclonal antibodies prepared against the purified Ca2+ + Mg2+-dependent ATPase of rabbit skeletal muscle sarcoplasmic reticulum was found to cross-react with calsequestrin and with a series of other Ca2+-binding proteins and their proteolytic fragments. Its cross-reactivity was enhanced in the presence of EGTA and diminished in the presence of Ca2+. Its lack of cross-reactivity with proteins that do not bind Ca2+ suggests that it has specificity for antigenic determinants that make up the Ca2+-binding sites in several Ca2+-binding proteins including the Ca2+ + Mg2+-dependent ATPase.

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Authors and Affiliations

  1. Banting and Best Department of Medical Research, University of Toronto, C.H. Best Institute, 112 College Street, M5G 1L6, Toronto, Ontario, Canada

    Elizabeth Zubrzycka-Gaarn, Glen MacDonald, Laurie Phillips & David H. MacLennan

  2. Department of Anatomy (Histology), University of Toronto, M5G 1L6, Toronto, Ontario, Canada

    Annelise O. Jorgensen

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  1. Elizabeth Zubrzycka-Gaarn
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  2. Glen MacDonald
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  3. Laurie Phillips
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  4. Annelise O. Jorgensen
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  5. David H. MacLennan
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This paper is dedicated to the memory of Dr. David E. Green.

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Zubrzycka-Gaarn, E., MacDonald, G., Phillips, L. et al. Monoclonal antibodies to the Ca2+ + Mg2+-dependent ATPase of sarcoplasmic reticulum identify polymorphic forms of the enzyme and indicate the presence in the enzyme of a classical high-affinity Ca2+ binding site. J Bioenerg Biomembr 16, 441–464 (1984). https://doi.org/10.1007/BF00743238

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  • DOI: https://doi.org/10.1007/BF00743238

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