Results and conclusions
The biochemical analyses of the total enzymatic activities of CK. PGAM in innervated cultures with advanced morphological maturation showed a progressive increase up to 60 days after innervation, fetal isozyme patterns of CK-BB and PGAM-BB at early stage of innervation and almost complete transition of CK and PGAM from BB to MM isozymes in more advanced stage of innervation (Figs 1, 2). Time course of G6PD activity in parallel cultures showed a higher activity in early stages of myogenesis(myoblastmyotube: 121.4+/-10 nM/min/mg prot) and in early phase of innervation (30 days after innervation: 109.66+/-26.10 nM/min/mg prot) with a decline of activity in advanced stage of innervation (60 days after innervation: 82.33+/-36.55 nM/min/mg prot) A progressive increase of AM activity in mid (30 days after innervation: 1623.66+/-10.96 pM/min/mg prot) and late stage of innervation (45 days after innervation: 2150.33+/-568.27 pM/min/mg prot) in comparison with aneural (536+/-107.39 pM/min/mg prot) was also found our study suggests these enzymes as biochemical markers of functional innervation of cultured human muscle fibers.
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References
Martinuzzi A., Askanas V. and Engel W.K. (1990) Paralysis of innervated cultured human muscle fibers affects enzymes differentially J. Neurochem. 54 223–229.
Meola, G., Velicogna, M., Sertorelli, S., Santilli, I. and Scarlato, G. (1989) Early and persistent successful functional innervation in human muscle cocultured with fetal mouse spinal cord complex. Europ. J. Neurosci. (suppl. 2). 229.
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Meola, G., Velicogna, M., Brigato, C. et al. CK-MM PGAM-MM G6PD and am biochemical markers of functional innervated cultured human muscle fibers. Cytotechnology 5 (Suppl 1), 176–177 (1991). https://doi.org/10.1007/BF00736842
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DOI: https://doi.org/10.1007/BF00736842