Summary
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1.
The histone complement of oligodendrocyte chromatin at different stages of brain development was studied after acid extraction of nuclei.
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2.
HCl-soluble proteins were analyzed by different electrophoretic techniques.
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3.
Our results show an increase in the concentration of histone H1° with differentiation.
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4.
The increase in H1° is accompanied by a concomitant decrease in the total amount of the ubiquitinated form of histone H2A (A24).
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References
Brown, I. R. (1978). Postnatal appearance of short DNA repeat length in neurons of the cerebral cortex.Biochem. Biophys. Res. Commun. 84285–292.
Cole, R. D. (1984). A minireview of microheterogeneity in H1 histone and its possible significance.Anal. Biochem. 13624–30.
Di Liegro, I., Salemi, G., and Cestelli, A. (1985). A low repeat length in oligodendrocyte chromatin.J. Neurochem. 451006–1012.
Gjerset, R., Gorka, C., Hasthorpe, S., Lawrence, J. J., and Eisen, H. (1982). Developmental and hormonal regulation of protein H1° in rodents.Proc. Natl. Acad. Sci. USA 792333–2337.
Goldknopf, I. L., Taylor, C. W., Baum, R. M., Yeoman, L. C., Olson, M. O. J., Prestayko, A. W., and Busch, H. (1975). Isolation and characterization of protein A24, a “histone-like” non-histone chromosomal protein.J. Biol. Chem. 2507182–7187.
Isenberg, I. (1979). Histones.Annu. Rev. Biochem. 48159–191.
Jaeger, A. W., and Kuenzle, C. C. (1982). The chromatin repeat length of brain cortex and cerebellar neurones changes concomitant with terminal differentiation.EMBO J. 1811–816.
Katovich Hurley, C. (1977). Electrophoresis of histones: A modified Panyim and Chalkley system for slab gels.Anal. Biochem. 80624–626.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227680–685.
Levinger, L., and Varshavsky, A. (1982). Selective arrangement of ubiquitinated and D1 proteincontaining nucleosomes within theDrosophila genome.Cell 28375–385.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. (1951). Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193265–275.
McGhee, J. D., Rau, D. C., Charney, E., and Felsenfeld, G. (1980). Orientation of the nucleosome within the higher order structure of chromatin.Cell 2287–96.
Moorman, A. F. M., de Boer, P. A. J., Charles, R., and Lamers, W. H. (1987). The histone H1°/H5 variant and terminal differentiation of cells during development ofXenopus laevis.Differentiation 35100–107.
Mori, S., and Leblond, C. P. (1970). Electron microscopic identification of three classes of oligodendrocytes and a preliminary study of their proliferative activity in the corpus callosum of young rats.J. Comp. Neurol. 1391–30.
Norton, W. T. (1984).Oligodendroglia, Plenum Press, New York.
Pehrson, J., and Cole, R. D. (1980). Histone H1° accumulates in growth inhibited cultured cells.Nature 28543–44.
Pehrson, J., and Cole, R. D. (1982). Histone H1 subfractions and H1° turnover at different rates in non-dividing cells.Biochemistry 21456–460.
Perkins, P. S., and Young, R. W. (1987). Comparison of histones in retina and brain nuclei from newborn and adult mice.Dev. Brain Res. 33161–168.
Piña, B., Martinez, P., Simon, L., and Suau, P. (1984). Differential kinetics of histone H1° accumulation in neuronal and glial cells from rat cerebral cortex during postnatal development.Biochem. Biophys. Res. Commun. 123697–702.
Risley, M. S., and Eckhardt, R. A. (1981). H1 histone variants inXenopus laevis.Dev. Biol. 8479–87.
Roche, J., Gorka, C., Goeltz, P., and Lawrence, J. J. (1985). Association of histone H1° with a gene repressed during liver development.Nature 314197–198.
Snyder, D. S., Raine, C. S., Farooq, M., and Norton, W. T. (1980). The bulk isolation of oligodendroglia from whole rat forebrain: A new procedure using physiologic media.J. Neurochem 341614–1631.
Thoma, F. (1988). The role of histone H1 in nucleosomes and chromatin fibers. InArchitecture of Eukaryotic Genes (G. Kahl, Ed.), VCH, Weinheim, pp. 163–185.
Thoma, F., Koller, T., and Klug, A. (1979). Involvement of histone H1 in the organization of the nucleosome and the salt-dependent superstructures of chromatin.J. Cell Biol. 83403–427.
Thomas, J. O., and Thompson, R. J. (1977). Variation in chromatin structure in two cell types from the same tissue: A short DNA repeat length in cerebral cortex neurons.Cell 10633–640.
Thomas, J. O., Rees, C., and Pearson, E. C. (1985). Histone H5 promotes the association of condensed chromatin fragments to give pseudo-higher-order structures.Eur. J. Biochem. 147143–151.
Weintraub, H. (1978). The neucleosomal repeat length increases during erythropoiesis in the chick.Nucleic Acids Res. 51179–1188.
Whatley, S. A., Hall, C., and Lim, L. (1981). Chromatin organization in the rat hypothalamus during early development.Biochem. J. 196115–119.
Wunsch, A. M., Haas, A. L., and Lough, J. (1987). Synthesis and ubiquitination of histones during myogenesis.Dev. Biol. 11985–93.
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Di Liegro, I., Cestelli, A. The relative proportion of H1° and A24 is reversed in oligodendrocytes during rat brain development. Cell Mol Neurobiol 10, 267–274 (1990). https://doi.org/10.1007/BF00734579
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DOI: https://doi.org/10.1007/BF00734579