Summary
The effects of chronic (3 to 8.5 months) smoke inhalation from cigarettes laced with 0.1, 1.0 and 10.0 mg kaolin (hydrated aluminum silicate) per gram of tobacco on the morphological integrity of lungs and the pulmonary macrophage population were evaluated in young and old male C57BL/6 mice. Lacing procedures, monitored by determining aluminum content in acid-digested aliquots of tobacco via atomic absorption spectrometry (AAS), proved to be uniform. Amounts of aluminum in right lungs of young mice evaluated by AAS and of kaolin assessed by electron diffraction and polarizing light microscopy were larger in mice which inhaled smoke from cigarettes laced with more kaolin. A more pronounced increase in lung parenchymal tissue and decrease of alveolar space was observed in old mice subjected to smoke from cigarettes containing higher doses of kaolin than in similarly treated young animals. Concomitant with the above, the lung macrophage population did not increase as markedly in response to smoke inhalation in old mice nor did it increase in as clear a dose-response fashion to kaolin as it did in young animals. Further, the degree of ultrastructural alteration of the phagocytes in the old mice suggested impaired cell function. Plate-like material resembling kaolin crystals was most conspicuous in lung macrophages of mice which inhaled largest amounts of kaolin. Manifestations of abnormal aggregates of lymphocytes and macrophages correlated with kaolin dose inhaled in old mice but not in young animals. The reported observations indicate that 1) kaolin gains access to lungs via cigarette smoke inhalation, 2) macrophages are important in maintaining pulmonary homeostasis and 3) the inorganic compound kaolin appears to impede macrophage function, resulting in potentiation of lung abnormalities.
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References
Altekruse EB, Chaudhary BA, Pearson MG, Morgan WKC (1984) Kaolin dust contamination and pneumoconiosis at a kaolin mine. Thorax 39:436–441
Brody AR, Craighead JE (1975) Cytoplasmic inclusions in pulmonary macrophages of cigarette smokers. Lab Invest 32:125–132
Choux R, Pautrat G, Viallat J, Farisse P, Boutin C (1978) Inorganic cytoplasmic inclusions in alveolar macrophages. Arch Pathol Lab Med 102:79–83
Cogbill EC, Hobbs ME (1957) Transfer of metallic constituents of cigarettes to the main-stream smoke. Tobacco Sci 1:68–73
Elias JA, Rossman MD, Zurier RB, Daniele RP (1985) Human alveolar macrophage inhibition of lung fibroblast growth: A prostaglandin-dependent process. Am Rev Respir Dis 131:94–99
Elias JA, Zurier RB, Rossman MD, Berube ML, Daniele RP (1984) Inhibition of lung fibroblast growth by human lung mononuclear cells. Am Rev Respir Dis 130:810–816
Finger H, Heymer B, Wirsing von Konig CH, Emmerling P (1982) Macrophage function in senescence. Gerontology 28:223–232
Frasca JM, Auerbach O, Parks VR, Jamieson JD (1971) Electron microscopic observations on pulmonary fibrosis and emphysema in smoking dogs. Exp Mol Pathol 15:108–125
Gairola CG (1986) Free lung cell response of mice and rats to mainstream cigarette smoke exposure. Toxicol Applied Pharmacol 84:567–575
Goldstein B (1959) Animal experiments on important parameters of dust in relation to the production of fibrosis. In: Orenstein AJ (ed) Proceedings of Pneumoconiosis Conference. Little Brown Co, Boston, MA, pp 94–108
Griffith RB, Hancock R (1985) Simultaneous mainstream-sidestream smoke exposure systems I. Equipment and procedures. Toxicology 34:123–138
Griffith RB, Standafer S (1985) Simultaneous mainstream-sidestream smoke exposure systems II. The rat exposure system. Toxicology 35:13–24
Hopewell T (1980) Idiopathic pulmonary fibrosis, lymphoid interstitial pneumonia, hypersensitivity pneumonitis, and collagen-vascular disease. In: Hinshaw C, Murry JF (eds) Tobacco: Experimental and clinical studies. Saunders, Philadelphia, pp 819–852
King EJ, Harrison CV, Nagelschmidt G (1948a) The effects of kaolin on the lungs of rats. J Pathol Bacteriol 60:435–440
Kline WE, Fogler HS (1981) Dissolution of silicate materials by hydrofluoric acid. Ind Eng Chem Fundom 20:155–161
Langer AM, Selikoff IJ, Sastre A (1971) Chrysotile asbestos in the lungs of persons in New York City. Arch Environ Health 22:348–352
Lapenas DJ, Gale PN (1983) Kaolin pneumoconiosis. Arch Pathol Lab Med 107:650–653
Lapenas D, Gale P, Kennedy T, Rawlings W, Dietrich P (1984) Kaolin pneumoconiosis - Radiologic, pathologic, and mineralogic findings. Am Rev Respir Dis 130:282–288
Low RB, Leffingwell GM, Bulman CA (1980) Effects of kaolinite on amino acid transport and incorporation into protein by rabbit alveolar macrophages. Arch Environ Health 35:217–223
Matulionis DH (1975) Light and electron microscopie study of the effects of ZnSO4 on mouse nasal epithelium and subsequent responses. Anat Rec 183:63–81
Matulionis DH (1984a) Chronic cigarette smoke inhalation and aging in mice: 1. Morphologic and functional lung abnormalities. Exp Lung Res 7:237–256
Matulionis DH (1984b) Effects of cigarette smoke generated by different smoking machines on pulmonary macrophages of mice and rats. J Anal Toxicol 8:187–191
Matulionis DH (1986) Lung deformation and macrophage displacement in smoke-exposed and normal mice (Mus musculus) following different fixation procedures. Virchow Arch A [Pathol Anat] 410:49–56
Matulionis DH, Kimmel E, Diamond L (1985) Morphologic and physiologic response of lungs to steroid and cigarette smoke: An animal model. Environ Res 36:298–313
Matulionis DH, Simmerman LA (1985) Chronic cigarette smoke inhalation and aging in mice: 2. Quantitation of the pulmonary macrophage response. Exp Lung Res 9:309–326
Matulionis DH, Traurig H (1977)In situ response of lung macrophages and hydrolase activity to cigarette smoke. Lab Invest 37:314–325
Ruttner JR, Spycher MA, Siticher H (1973) The detection of etiologie agents in interstitial pulmonary fibrosis. Human Pathol 4:497–512
Sepulveda MJ, Vallyathan V, Attfield MD, Piacitelli L, Tucker JH (1983) Pneumoconiosis and lung function in a group of kaolin workers. Am Rev Respir Dis 127:231–235
Slonecker LE, Lim WC (1972) Effects of hydrocortisone on the cells in an acute inflammatory exudate. Lab Invest 27:123–128
Slonim, NB, Hamilton LH (1981) Respiratory physiology. Mosby, St. Louis, MO
Vallyathan V, Hahn LH (1985) Cigarette smoking and inorganic dust in human lungs. Arch Environ Health 40:69–73
Van Furth R, Blusse Van Oud Alblas A (1982) The current view on the origin of pulmonary macrophages. Pathol Res Pract 175:38–49
Weeks BA (1979) Effects of age and nutrition on macrophage function. J Reticuloendoth Soc 26:459–462
Weibel ER (1979) Stereological Methods. Practical methods for biological morphometry. Vol 1, Academic Press, New York, NY
White R, Kuhn C (1980) Effects of phagocytosis of mineral dusts on elastase secretion by alveolar and peritoneal exudative macrophages. Arch Environ Res 35:106–109
Yokel RA, Melograna JM (1983) A safe method to acid digest small samples of biological tissues for graphite furnace atomic absorption analysis of aluminum. Biol Tr Elem Res 5:225–237
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Matulionis, D.H., Yokel, o.A. Murine lung response to kaolin conveyed by cigarette smoke. Vichows Archiv A Pathol Anat 413, 227–237 (1988). https://doi.org/10.1007/BF00718615
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DOI: https://doi.org/10.1007/BF00718615