Summary
Twenty-four hours after nerve crush, the Schwann cell plasma membrane and subjacent outer layer of Schwann cell cytoplasm were examined by freeze-fracture in myelinated fibres from the rat and rabbit sciatic nerves. The irregular circumferential bands and longitudinal columns of cytoplasm which characterise the surface of the normal Schwann cell were diminished or had disappeared. Concomitantly, the membrane pores (of micropinocytotic vesicles or caveolae) which are normally present on the bands and columns were also lost. The loss of these specialised features is discussed in terms of the onset of cell dedifferentiation in association with cell division. The Schwann cell plasma membrane acquired a new feature: deep impressions of adjacent collagen fibres, present particularly on the cytoplasmic bands and columns and presumed to be related to a transient increase in fibre diameter.
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References
Abrahams P, Day A, Allt G (1979) Observations on the Schwann cell plasma membrane using freeze-fracture. J Anat 128:430–433
Abrahams P, Day A, Allt G (1980) Mammalian peripheral nerve-a difficult tissue to crack. Proc R Microsc Soc (in press)
Allt G (1976) Pathology of the peripheral nerve. In: Landon DN (ed) The peripheral nerve, chapt 13. Chapman and Hall, London
Bordi C (1979) Parlodian coating of highly fragile freeze-fracture replicas. Micron 10:139–140
Branton D, Bullivant S, Gilula NB, Karnovsky MJ, Moor H, Mühlethaler K, Northcote DH, Packer L, Satir G, Satir P, Speth V, Staehlin LA, Steere RL, Weinstein RS (1975) Freeze-etching nomenclature. Science 190:54–56
Bullivant S (1973) Freeze-etching and freeze-fracturing. In: Koehler JK (ed) Advanced techniques in biological electron microscopy. Springer, Berlin Heidelberg New York, pp 67–112
Day A, Ubee D (1980) Modifications and improvements to the Edwards freeze-fracture and etching accessory. Proc R Microsc Soc (in press)
Donat JR, Wisńiewski HM (1973) The spatio-temporal pattern of Wallerian degeneration in mammalian peripheral nerves. Brain Res 53:41–53
Friede RL, Bischhausen R (1978) The organisation of endoneural collagen in peripheral nerves as revealed with the scanning electron microscope. J Neurol Sci 38:83–88
Ghabriel MN, Allt G (1979) The role of Schmidt-Lanterman incisures in Wallerian degeneration. I. A quantitative teased fibre study. Acta Neuropathol (Berl) 48:83–93
Gershenbaum MR, Roisen FJ (1978) A scanning electron microscopic study of peripheral nerve degeneration and regeneration. Neuroscience 3:1241–1250
Kruger L, Stolinski C, Martin BGH, Gross MB (1979) Membrane specializations and cytoplasmic channels of Schwann cells in mammalian peripheral nerve as seen in freeze-fracture replicas. J Comp Neurol 186:571–602
Lubińska L (1961) Sedentary and migratory states of Schwann cells. Ex Cell Res [Suppl] 8:74–90
Lubińska L, Jastreboff P (1977) Early course of Wallerian degeneration in myelinated fibres of the rat phrenic nerve. Brain Res 130:47–63
Mugnaini E, Osen KK, Schnapp B, Friedrich VL (1977) Distribution of Schwann cell cytoplasm and plasmalemmal vesicles (caveolae) in peripheral myelin sheaths. An electron microscope study with thin sections and freeze-fracturing. J Neurocytol 6:647–668
Sandri C, Van Buren JM, Akert K (1977) Membrane morphology of the vertebrate nervous system. A study in freeze-etch technique. Prog Brain Res 46, Elsevier, Amsterdam
Satinsky D, Pepe FA, Liu CN (1964) The neurilemma cell in peripheral nerve degeneration and regeneration. Exp Neurol 9:441–451
Spencer PS, Lieberman AR (1971) Scanning electron microscopy of isolated peripheral nerve fibres. Normal surface structure and alterations proximal to neuromas. Z Zellforsch Mikros Anat 119:534–551
Thomas PK (1963) The connective tissue of peripheral nerve: an electron microscope study. J Anat 97:35–44
Thomas PK (1964) Changes in the endoneurial sheaths of peripheral myelinated nerve fibres during Wallerian degeneration. J Anat 98:175–182
Vial JD (1958) The early changes in the axoplasm during Wallerian degeneration. J Biophys Biochem Cytol 4:551–556
Webster H de F (1962) Transient focal accumulation of axonal mitochondria during the early stages of Wallerian degeneration. J Cell Biol 12:361–384
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Abrahams, P.H., Day, A. & Allt, G. Schwann cell plasma membrane changes induced by nerve crush. Acta Neuropathol 50, 85–90 (1980). https://doi.org/10.1007/BF00692856
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DOI: https://doi.org/10.1007/BF00692856