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Lysine transport across the mucosal border of the perfused midgut in the freshwater shrimp,Macrobrachium rosenbergii

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Summary

  1. 1)

    Unidirectional transmembrane transport of3H-lysine across the mucosal border of the midgut from the freshwater shrimp,Macrobrachium rosenbergii, has been examined using an in vitro perfusion procedure.

  2. 2)

    Influx of this amino acid appeared to take place through a high-affinity, carrier-mediated transport system displaying Michaelis-Menten kinetics (Kt=18.0±2.1 μM;J maxmc =0.50±0.06 nm/g min) and an apparent low-affinity mechanism that had a transfer rate which was a linear function of luminal lysine concentration. The latter system cannot be conclusively distinguished from simple diffusion at this time.

  3. 3)

    The high-affinity process was sodium-dependent (lithium adequately substituting for sodium) and inhibited by arginine and iodoacetic acid.

  4. 4)

    The apparent low-affinity transport mechanism displayed homoexchange diffusion and was sodium-independent, inhibited by arginine and unaffected by iodoacetic acid.

  5. 5)

    Entry of lysine by either process was unresponsive to NaCN, ouabain and alteration of luminal pH.

  6. 6)

    These results suggest that cationic amino acid influx in crustacean intestine appears similar to processes reported for these solutes from a large variety of cell types. The major difference found for the shrimp gut is the apparent adaptation of the high-affinity carrier process for very low lysine concentrations.

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Author notes

  1. Robert W. Brick

    Present address: Department of Wildlife and Fisheries Sciences, Texas A&M University, 77843, College Station, Texas, USA

Authors and Affiliations

  1. Hawaii Institute of Marine Biology, University of Hawaii, Box 1346, 96744, Kaneohe, Hawaii, USA

    Robert W. Brick & Gregory A. Ahearn

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  1. Robert W. Brick
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  2. Gregory A. Ahearn
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Contribution No. 539 from the Hawaii Institute of Marine Biology, Kaneohe, Hawaii

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Brick, R.W., Ahearn, G.A. Lysine transport across the mucosal border of the perfused midgut in the freshwater shrimp,Macrobrachium rosenbergii . J Comp Physiol B 124, 169–179 (1978). https://doi.org/10.1007/BF00689178

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