Summary
Five patients with various brain tumors received bromodeoxyuridine (BrdU), 150–200 mg/m2 i.v.; at the time of craniotomy. Biopsied materials were fixed in 70% ethanol, sectioned, denatured with hydrochloric acid, and reacted with monoclonal antibodies against BrdU. Immunofluorescence and immunocytochemical methods were used to visualize BrdU-labeled nuclei. Our results showed that both methods demonstrated BrdU-labeled nuclei satisfactorily in tissue sections. Thus, BrdU can be used to measure the proliferative potential of human tumors in situ.
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References
Bagshaw MA, Doggett RLS, Smith KC, Kaplan HS, Nelson TS (1967) Intraarterial 5-bromodeoxyuridine and x-ray therapy. Am J Roentgenol 99:886–894
Chigasaki H (1963) Studies on the DNA synthesis function of glial cells by means of3H-thymidine microradioautography. Brain Nerve 15:767–781
Dean NP, Dolbeare F, Gratzner H, Rice GC, Gray JW (1984) Cell cycle analysis using a monoclonal antibody to BrdU. Cell Tissue Kinet 17:427–436
Dolbeare F, Gratzner H, Pallavicini MG, Gray JW (1983) Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci (USA) 80:5573–5577
Gratzner HG (1982) Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 218:474–476
Hoshino T (1977) Therapeutic implications of brain tumor cell kinetics. Natl Cancer Inst Monogr 46:29–35
Hoshino T (1979) The cell kinetics of gliomas: its prognostic value and therapeutic implications. In: Paoletti PM, Walker MD, Butti G, Knerich R (eds) Multidisciplinary aspects of brain tumor therapy. Elsevier/North Holland, Amsterdam, pp 105–112
Hoshino T, Barker M, Wilson CB, Boldrey EB, Fewer D (1972) Cell kinetics of human gliomas. J Neurosurg 37:15–26
Hoshino T, Sano K (1969) Radiosensitization of malignant brain tumors with bromouridine (thymidine analogue). Acta Radiol Ther Phys Biol 8:15–21
Hoshino T, Townsend JJ, Muraoka I, Wilson CB (1980) An autoradiographic study of human gliomas: growth kinetics of anaplastic astrocytoma and glioblastoma multiforme. Brain 103:967–984
Hoshino T, Wilson CB (1979) Cell kinetic analyses of human malignant brain tumors (gliomas). Cancer 44:956–962
Hoshino T, Wilson CB, Ellis WG (1975) Gemistocytic astrocytes in gliomas: an autoradiographic study. J Neuropathol Exp Neurol 34:263–281
Kinsella TJ, Russo A, Mitchell JB, Rowland J, Jenkins J, Schwade J, Myers CE, Collins JM, Speyer J, Kornblith P, Smith B, Kufta C, Glatstein EA (1984) A phase I study of intermittent intravenous bromodeoxyuridine (BUdR) with conventional fractionated irradiation. Int J Radiat Oncol Biol Phys 10:69–76
Kury G, Carter HW (1965) Autoradiographic study of human nervous system tumors. Arch Pathol 80:38–42
Mitchell JB, Kinsella TJ, Russo A, McPherson S, Rowland J, Kornblith P, Gladstein E (1983) Radiosensitization of hematopoietic precursor cells (CFUc) in glioblastoma patients receiving intermittent intravenous infusions of bromodeoxyuridine (BUdR). Int J Radiat Oncol Biol Phys 9:457–463
Morstyn G, Hsu SM, Kinsella T, Gratzner H, Russo A, Mitchell JB (1983) Bromodeoxyuridine in tumors and chromosomes detected with a monoclonal antibody. J Clin Invest 72: 1844–1850
Nagashima T, Hoshino T (1985) Rapid detection of S-phase cells by anti-bromodeoxyuridine monoclonal antibody in 9L brain tumor cells in vitro and in situ. Acta Neuropathol (Berl) (in press)
Russo A, Gianni L, Kinsella TJ, Klecker RW, Jenkins J, Rowland J, Glatstein E, Mitchell JB, Collins J, Myers C (1984) Pharmacological evaluation of intravenous delivery of 5-bromodeoxyuridine to patients with brain tumors. Cancer Res 44:1702–1705
Sano K, Hoshino T, Nagai M (1968) Radiosensitization of brain tumor cells with a thymidine analogue (bromouridine). J Neurosurg 28:530–538
Szybalski W (1974) X-ray sensitization by halopyrimidines. Cancer Chemother Rep 58:539–557
Tannock IF (1978) Cell kinetics and chemotherapy: A critical review. Cancer Treat Rep 62:1117–1133
Tym R (1969) Distribution of cell doubling times in vivo human cerebral tumors. Surg Forum 20:445–447
Van Dilla MA, Trujillo TT, Mullaney PF, Coulter JR (1969) Cell microfluorometry: A method for rapid fluorescence measurement. Science 163:1213–1214
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Supported by grant PDT-159 from the American Cancer Society and by grant CA 13525 from the National Cancer Institute
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Nagashima, T., DeArmond, S.J., Murovic, J. et al. Immunocytochemical demonstration of S-phase cells by anti-bromodeoxyuridine monoclonal antibody in human brain tumor tissues. Acta Neuropathol 67, 155–159 (1985). https://doi.org/10.1007/BF00688136
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DOI: https://doi.org/10.1007/BF00688136