Skip to main content
Log in

Optimal conditions for breaking medically important yeasts by an inexpensive and simple method

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

Conditions for breaking various medically important yeasts using glass beads, 30 ml Corex centrifuge tubes, and a Vortex mixer were determined. From 75–95% ofCandida hyphal cells and all species of yeasts exceptSporothrix schenckii were broken when 10 g of 0.45–0.50 mm glass beads, 50–300 mg of wet cells in 5 ml of buffer, and 90 s of vortexing were employed. Yeasts ofSporothrix schenckii broke more efficiently when 0.25–0.30 mm beads were used.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Chaffin, W. L., Barton, R. A., Jacobson, E. L. & Jacobson, M. K., 1979. Nicotinamide adenine dinucleotide metabolism in Candida albicans. J. Bacteriol. 139: 883–888.

    Google Scholar 

  2. Lipinski, C., Ferro A. J. & Mills, D., 1976. Macromolecular synthesis in a mutant of Saccharomyces cerevisiae inhibited by S-adenosylmethionine. Molec. gen. Genet. 144: 301–306.

    Google Scholar 

  3. Ribi, E. & Milner, K. C., 1967. Description of Bacteria and Preparation of Cell Walls, p. 13–28. In Meth. Immunol. Immunochem., vol. 1, C. A. Williams and M. W. Chase (Eds.) Acad. Press, Inc., N.Y.

    Google Scholar 

  4. Richards, W. C. & Takai, S., 1979. A microfuge tube rechnique for disrupting microquantities of fungal spores. Anal. Biochem. 100: 240–243.

    Google Scholar 

  5. Van Etten, J. L. & Freer, S. N., 1978. Simple procedure for disruption of fungal spores. Appl. Environ. Microbiol. 35: 622–623.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hazen, K.C., Cutler, J.E. Optimal conditions for breaking medically important yeasts by an inexpensive and simple method. Mycopathologia 80, 113–116 (1982). https://doi.org/10.1007/BF00641187

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00641187

Keywords

Navigation