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Induction of cytochrome P-448 iso-enzymes and related glucuronyltransferases in the human liver by cigarette smoking

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Summary

Human liver 10–20 mg from biopsy samples removed for diagnostic purposes from selected patients has been used for the preparation of microsomes. The activity of certain mono-oxygenases and UDP-glucuronyltransferases, as well as certain other enzymes involved in drug metabolism, has been determined using different substates as indicators. Smokers oxidized benzo(a)pyrene and 7-ethoxyresorufin and also conjugatedα-naphthol at a significantly increased rate compared to non-smokers. This indicates induction of certain mono-oxygenases and glucuronyltransferases which differs from the induction produced by phenobarbital. The findings can account for the reduced activity and increased elimination rate of certain drugs prescribed to smokers.

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Authors and Affiliations

  1. Medizinische Universitätsklinik, Tübingen, Federal Republic of Germany

    R. Fleischmann & U. Stärz

  2. Institut für Toxikologie der Universität Tübingen, Tübingen, Federal Republic of Germany

    H. Remmer

Authors
  1. R. Fleischmann
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  2. H. Remmer
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  3. U. Stärz
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Dedicated to Professor Dr. med. Helmut Kewitz on the occasion of his 65th birthday

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Fleischmann, R., Remmer, H. & Stärz, U. Induction of cytochrome P-448 iso-enzymes and related glucuronyltransferases in the human liver by cigarette smoking. Eur J Clin Pharmacol 30, 475–480 (1986). https://doi.org/10.1007/BF00607963

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  • DOI: https://doi.org/10.1007/BF00607963

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