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Transport ofl-lysine by rat renal brush border membrane vesicles

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

l-3H-lysine uptake into brush border membrane vesicles was measured by a rapid filtration technique. A significant binding ofl-lysine at the vesicle interior was observed. Extrapolating initial linear uptake to zero incubation time did not indicate binding of the amino acid to the external membrane surface.

Sodium stimulated thel-lysine uptake specifically. Experiments in the presence of potassium/valinomycin induced diffusion potentials, and experiments with a potential sensitive fluorescent dye documented an electrogenic uptake mechanism forl-lysine only in the presence of sodium. Sodium independent uptake proceeds via an electroneutral pathway. Transstimulation experiments show carrier mediated uptake in the presence and absence of sodium. An outwardly directed proton-gradient stimulatedl-lysine uptake in the presence and absence of sodium.

Saturation ofl-lysine uptake was observed in the presence and absence of sodium. In the absence of sodium,l-lysine uptake was inhibited byl-arginine,l-cystine,l-phenylalanine andl-methionine. The sodium dependent uptake was inhibited byl-arginine andl-cysteine; small inhibition byl-phenylalanine was observed. In the presence or absence of sodium,l-lysine uptake was inhibited neither byd-lysine nor byl-glutamic acid.

These results document carrier mediated transport ofl-lysine via (a) transport mechanism(s) not obligatory requiring sodium.

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Abbreviations

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethane sulfonic acid

Tris:

Tris(hydroxymethy)aminomethane

EGTA:

ethyleneglycol-bis-(β-aminoethyl-ether)-N,N′-tetraacetic acid

diamide:

azodicarboxylic acid[bisdimethylamide]

FCCP:

carbonyl cyanide p-trifluoromethoxyphenylhydrazone

MES:

2-(N-morpholino) ethanesulfonic acid

References

  • Barrett PQ, Aronson PS (1982) Glucose and alanine inhibition of phosphate transport in renal microvillous membrane vesicles. Am J Physiol 242:F126-F131

    Google Scholar 

  • Beck JC, Sacktor B (1978) Membrane potential-sensitive fluorescence changes during Na+-dependentd-glucose transport in renal brush border membrane vesicles. J Biol Chem 253:7158–7162

    Google Scholar 

  • Berner W, Kinne R, Murer H (1976) Phosphate transport into brush border membrane vesicles isolated from rat small intestine. Biochem J 160:467–474

    Google Scholar 

  • Biber J, Stieger B, Haase W, Murer H (1981) A high yield preparation for rat kidney brush border membranes. Different behaviour of lysosomal markers. Biochim Biophys Acta 647:169–176

    Google Scholar 

  • Biber J, Stange G, Stieger B, Murer H (1983) Transport ofl-cystine by rat renal brush border membrane vesicles. Pflügers Arch 396:335–341

    Google Scholar 

  • Burckhardt G, Murer H (1981) A cyanine dye as indicator of membrane electrical potential differences in brush border membrane vesicles. Studies with K+-gradients and Na+/amino acid cotransport. Proc Int Cong Physiol, Budapest, vol 11:409–418

    Google Scholar 

  • Burckhardt G, Kinne R, Stange G, Murer H (1980) The effects of potassium and membrane potential on sodium dependentl-glutamic acid uptake. Biochim Biophys Acta 599:191–201

    Google Scholar 

  • Busse D (1978) Transport ofl-arginine in brush border vesicles derived from rabbit kidney cortex. Arch Biochem Biophys 191:551–560

    Google Scholar 

  • Frömter E (1979) Solute transport across epithelia: What ca we learn from micropuncture studies on kidney tubules. J Physiol 288:1–31

    Google Scholar 

  • Haase W, Schäfer A, Murer H, Kinne R (1978) Studies on the orientation of brush border membrane vesicles. Biochem J 172:57–62

    Google Scholar 

  • Hammerman MR (1982) Na+-independentl-arginine transport in rabbit renal brush border membrane vesicles. Biochim Biophys Acta 685:71–77

    Google Scholar 

  • Hilden SA, Sacktor B (1981)l-Arginine uptake into renal brush border membrane vesicles. Arch Biochem and Biophys 210:289–297

    Google Scholar 

  • Kessler M, Tannenbaum V, Semenza G (1978) A simple apparatus for performing short time (1–2s) uptake measurements in small volumes; its application tod-glucose transport studies in brush border vesicles from rabbit jejunum and ileum. Biochim Biophys Acta 509:348–359

    Google Scholar 

  • Mircheff AK, Kippen J, Hirayama B, Wright EM (1982) Delineation of sodium stimulated amino acid transport pathways in rabbit kidney brush border vesicles. J Membr Biol 64:113–122

    Google Scholar 

  • Murer H, Sigrist-Nelson H, Hopfer U (1975) On the mechanism of sugar and amino acid interaction in intestinal transport. J Biol Chem 250:7392–7396

    Google Scholar 

  • McNamara PD, Pepe LM, Segal S (1981) Cystine uptake by rat renal brush border vesicles. Biochem J 194:443–449

    Google Scholar 

  • Rosenberg LE, Albrecht J, Segal S (1967) Lysine transport in human kidney. Evidence for two systems. Science 155:1426–1428

    Google Scholar 

  • Sacktor B, Lepor N, Schneider EG (1981) Stimulation of efflux ofl-glutamate from renal brush border membrane vesicles by extravesicular potassium. Bioscience Reports 1:709–713

    Google Scholar 

  • Samarzija J, Frömter E (1982) Electrophysiological analysis of rat renal sugar and amino acid transport. IV. Basic amino acids. Pflügers Arch 393:210–214

    Google Scholar 

  • Schäfer JA, Barfuss DW (1980) Membrane mechanism for transepithelial amino acid absorption and secretion. Am J Physiol 238:F335-F346

    Google Scholar 

  • Schneider EG, Sacktor B (1980) Sodium gradient dependentl-glutamate transport in renal brush border membrane vesicles. Effect of an intravesicular > extravesicular potassium gradient. J Biol Chem 255: 7645–7649

    Google Scholar 

  • Schneider EG, Hammerman MR, Sacktor B (1980) Sodium gradient dependentl-glutamate transport in renal brush border membrane vesicles. Evidence for an electroneutral mechanism. J Biol Chem 255:7650–7656

    Google Scholar 

  • Segal S, Thier SO (1973) Renal handling of amino acids. In: Orloff J, Berliner RW (eds) Handbook of physiology, vol 8, renal physiology. Williams & Wilkins, Baltimore, pp 653–676

    Google Scholar 

  • Segal S, McNamara PD, Pepe LM (1977) Transport interaction of cystine and dibasic amino acids in renal brush border vesicles. Science 197:169–171

    Google Scholar 

  • Sigrist-Nelson K, Murer H, Hopfer U (1975) Active alanine transport in isolated brush border membranes. J Biol Chem 250:5674–5680

    Google Scholar 

  • Silbernagl S (1975) Cycloleucine (1-amino-cyclopentane carboxylic acid): tubular reabsorption and inhibitory effect on amino acid transport in the rat kidney (microperfusion experiments). Pflügers Arch 353:241–253

    Google Scholar 

  • Silbernagl S (1981) Renal transport of amino acids and oligopeptides. In. Greger R, Lang F, Silbernagl S (eds) Renal transport or organic substances. Springer, Berlin Heidelberg New York, pp 93–117

    Google Scholar 

  • Silbernagl S, Deetjen P (1972) The tubular reabsorption ofl-cystine andl-cysteine. A common transport system withl-arginine or not? Pflügers Arch 337:277–284

    Google Scholar 

  • Silbernagl S, Deetjen P (1973) Molecular specificity of thel-arginine reabsorption mechanism. Microperfusion studies in the proximal tubule of rat kidney. Pflügers Arch 340:325–334

    Google Scholar 

  • Spears G, Sneyd JGT, Loten EG (1971) A method for deriving kinetic constants for two enzymes acting on the same substrate. Biochem J 125:1149–1151

    Google Scholar 

  • Stieger B, Stange G, Biber J, Murer H (1982) Transport ofl-cysteine by rat renal brush border membrane vesicles. J Membr Biol [in press]

  • Thierry J, Poujeol P, Ripoche P (1981) Interactions between Na+-dependent uptake ofd-glucose, phosphate andl-alanine in rat renal brush border membrane vesicles. Biochem Biophys Acta 647: 203–210

    Google Scholar 

  • Turner ST, Dousa TP (1982) Differences in transport capacity for phosphate (Pi) of luminal brush border membrane (BBM) prepared from subcapsular (SC) and juxtamedullary (JM) cortex. Kidney Int 21: 141

    Google Scholar 

  • Turner RJ, Moran A (1982) Heterogeneity of sodium dependentd-glucose transport sites along the proximal tubule: evidence from vesicle studies. Am J Physiol 242:F406-F414

    Google Scholar 

  • Ullrich KJ (1979) Sugar, amino acid and Na+ cotransport in the proximal tubule. Ann Rev Physiol 41:181–195

    Google Scholar 

  • Ullrich KJ, Rumrich G, Klöss S (1974) Sodium dependence of the amino acid transport in the proximal convolution of the rat kidney. Pflügers Arch 351:49–60

    Google Scholar 

  • Wright SH, Krasne SK, Kippen J, Wright EM (1981) Na+-dependent transport of tricarboxylic acid cycle intermediates by renal brush border membranes. Effects on fluorescence of a potential sensitive cyanine dye. Biochim Biophys Acta 640:767–778

    Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, University of Zürich, Rämistrasse 69, CH-8028, Zürich, Switzerland

    B. Stieger, G. Stange, J. Biber & H. Murer

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  1. B. Stieger
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  2. G. Stange
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  3. J. Biber
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  4. H. Murer
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Stieger, B., Stange, G., Biber, J. et al. Transport ofl-lysine by rat renal brush border membrane vesicles. Pflugers Arch. 397, 106–113 (1983). https://doi.org/10.1007/BF00582047

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  • DOI: https://doi.org/10.1007/BF00582047

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