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Affinity chromatography of carboxylic proteinases on a polymeric sorbent containing gramicidin-C as ligand

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Abstract

A new polymeric sorbent for proteinases has been synthesized by the radical copolymerization of N-vinylpyrrolidone, bis-Nδ-acryloylgramicidin C, and N,N′-methylenebisacrylamide. Biospecific chromatography on the new sorbent has enabled an industrial preparation of procine pepsin to be purified by a factor of 2.5. With the aid of the new sorbent, a carboxylic proteinase has been isolated from the industrial preparation Tsellolignorin with a 15-fold purification factor.

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Literature cited

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Authors
  1. G. N. Balandina
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  2. G. I. Lavrenova
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  3. L. D. Uzhinova
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  4. E. A. Chekhovskikh
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  5. N. A. Platé
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  6. V. M. Stepanov
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Additional information

M. V. Lomonosov State University. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 215–218, March–April, 1986.

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Balandina, G.N., Lavrenova, G.I., Uzhinova, L.D. et al. Affinity chromatography of carboxylic proteinases on a polymeric sorbent containing gramicidin-C as ligand. Chem Nat Compd 22, 200–203 (1986). https://doi.org/10.1007/BF00574739

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  • DOI: https://doi.org/10.1007/BF00574739

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