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Regulation and expression of transforming growth factor type-ß during early mammalian development

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Abstract

We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-β) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-β activity increases approximately 25–100% when the mouse EC cell line, F9, is induced to differentiate with retinoic acid (RA). Interestingly, the increased TGF-β activity reflects the induction of TGF-β2 secretion following differentiation of both F9 EC cells and the human EC cell line, NT2/D1. Using the technique of reverse transcription-polymerase chain reaction (RT-PCR), we have verified that differentiation induces the expression of TGF-β2 as well as a distant member of the TGF-β family, Vgr-1. Transcripts for TGF-β2 and Vgr-1 were readily detected in the differentiated cells of F9 and PC-13 but not in their undifferentiated counterparts. Moreover, TGF-β2 mRNA was readily detected in NT2/D1 cells following differentiation. In addition, transcripts for TGF-β2 were detected by RT-PCR in mouse morulae, preimplantation blastocysts and cultured blastocysts. Based on the data presented, it appears that the expression of both TGF-β2 and Vgr-1 is closely associated with the induction of differentiation during early development.

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Abbreviations

CS:

Calf Serum

EC:

Embryonal Carcinoma

FBS:

Fetal Bovine Serum

RA:

Retinoic Acid

RT-PCR:

Reverse Transcription-Polymerase Chain Reaction

TGF-β:

Transforming Growth Factor type-beta

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Authors and Affiliations

  1. Eppley Institute for Cancer Research and Allied Diseases, University of Nebraska Medical Center, 42nd and Dewey Avenue, 68198-6805, Omaha, NE, USA

    David Kelly, Wendy J. Campbell, Jay Tiesman & Angie Rizzino

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  1. David Kelly
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  2. Wendy J. Campbell
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  4. Angie Rizzino
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Kelly, D., Campbell, W.J., Tiesman, J. et al. Regulation and expression of transforming growth factor type-ß during early mammalian development. Cytotechnology 4, 227–242 (1990). https://doi.org/10.1007/BF00563783

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  • DOI: https://doi.org/10.1007/BF00563783

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