Summary
Monoclonal antibodies were produced against monkey lung lavage fluid by using a mouuse hybridoma technique. One monoclonal antibody, KP8D4, specifically reacted with basal cells in human bronchial epithelia by immunohistological staining of acetone-fixed, frozen sections and it recognized a protein with an apparent molecular weight of 84000, as determined by gel immunoblotting. The distribution of this protein was immunohistochemically examined in various human tissues (lung, tongue, esophagus, stomach, intestine, liver, pancreas, salivary gland, spleen, thymus, heart, aorta, vena cava, prostate, breast, kidney, urinary bladder, thyroid, brain, skin, striated muscle) and various tissues of rats, rabbits and pigs. The results showed a specific affinity of KP8D4 to basal cells of stratified epithelia in the various human and rabbit tissues. This antibody may be a useful tool for studies of normal development and diverse pathological disorders.
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References
Banks-Schlegel SP (1982) Keratin alterations during embryonic epidermal differentiation: A presage of adult epidermal maturation. J Cell Biol 93:551–559
Blenkinsopp WK (1967) Proliferation of respiratory tract epithelium in the rat. Exp Cell Res 46:144–154
Brawer MK, Peehl DM, Stamey TA, Bostwick DG (1985) Keratin immunoreactivity in the benign and neoplastic human prostate. Cancer Res 45:3663–3667
Caselitz J, Walther B, Wastrow J, Seifert G, Weber K, Osborn M (1986) A monoclonal antibody that detects myoepithelial cells in exocrine glands, basal cells in other epithelia and basal and suprabasal cells in certain hyperplastic tissues. Virchows Arch [Pathol] Anat 409:725–738
Cohen S, Elliott GA (1962) The stimulation of epidermal keratinization by a protein isolated from the submaxillary gland of the mouse. J Invest Dermatol 40:1–5
Dairkee SH, Blayney C, Smith HS, Hackett AJ (1985) Monoclonal antibody that defines human myoepithelium. Proc Natl Acad Sci USA 82:7409–7413
Franke WW, Schiller DL, Moll R, Winter S, Schmid E, Engelbrecht I (1981) Diversity of cytokeratins: Differentiation specific expression of cytokeratin polypepties in epithelial cells and tissues. J Mol Biol 153:933–959
Fuchs E, Green H (1980) Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell 19:1033–1042
Gatter KC, Brown G, Trowbridge IS, Woolston R-E, Mason DY (1983) Transferrin receptors in human tissues: their distribution and possible clinical relevance. J Clin Pathol 36:539–545
Gould PR, Barter RA, Papadimitriou JM (1979) An ultrastructural, cytochemical, and autoradiographic study of the mucous membrane of the human cervical canal with reference to subcolumnar basal cells. Am J Pathol 95:1–16
Gusterson B, Cowley G, Smith JA, Ozanne B (1984) Cellular localization of human epidermal growth factor receptor. Cell Biol Int Rep 8:649–658
Hsu S-M, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Johnson LD (1969) The histopathological approach to early cervical neoplasia. Obstet Gynecol Surv 24:735–767
Klein-Szanto AJP, Topping DC, Heckman CA, Nettesheim P (1980) Ultrastructural characteristics of carcinogen-induced dysplastic changes in tracheal epithelium. Am J Pathol 98:83–100
Köhler G, Milstein C (1976) Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol 6:511–519
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lang AB, Odermatt BF, Ruettner JR (1986) Monoclonal antibodies to human cytokeratins: Application to various epithelial and mesothelial cells. Exp Cell Biol 54:61–72
Morinaga S, Nakajima T, Shimosato Y (1987) Normal and neoplastic myoepithelial cells in salivary glands: An immunohistochemical study. Hum Pathol 18:1218–1226
Nagle RB, Bocker W, Davis JR, Heid HW, Kaufmann M, Lucas DO, Jarasch E-D (1986) Characterization of breast carcinomas by two monoclonal antibodies distinguishing myoepithelial from luminal epithelial cells. J Histochem Cytochem 34:869–881
Nasiell M, Auer G, Kato H (1987) Cytological studies in man and aminals on development of bronchogenic carcinoma. In: McDowell EM (ed) Lung carcinomas. Churchill Livingstone, Edinburgh London, pp 207–242
Nimi T, Imaizumi M, Abe T, Haimoto H, Nagura H (1987) Immunohistochemical characteristics of proliferative and metaplastic lesions in bronchial mucosa. Am J Clin Pathol 88:545–551
Pallesen G, Nielsen S, Cells JE (1987) Characterization of a monoclonal antibody (BG3C8) that reacts with basal cells of stratified epithelia. Histopathology 11:591–601
Pavlovitch JH, Delezoide AL, Didierjean L, Saurat JH, Pfister A (1984) Skin calcium-binding protein in squamous metaplasia of human uterine cervix. Am J Pathol 114:454–460
Reagan JW, NG ABP, Wentz WB (1969) Concepts of genesis and development in early cervical neoplasia. Obstet Gynecol Surv 24:860–874
Seligman PA, Schleicher RB, Allen RH (1979) Isolation and characterization of the transferrin receptor from human placenta. J Biol Chem 254:9943–9946
Stegner HE, Beltermann R (1969) Die Elektronemikroskopie des Cervixdrüsenepithels und des sog. Reservezellen. Arch Gynecol 207:480–504
Tatemoto Y, Fukui S, Oosumi H, Horike H, Mori M (1987) Expression of keratins during experimentally induced carcinogenesis in hamster cheek pouch visualized polyclonal and monoclonal antibodies. Histochemistry 86:445–452
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Weikel W, Wagner R, Moll R (1987) Characterization of subcolumnar reserve cells and other epithelia of human uterine cervix. Virchows Arch [Cell Biol] 54:98–110
Woodcock-Mitchell J, Eichner R, Nelson WG, Sun T-T (1982) Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 95:580–588
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Maeda, K., Sueishi, K. A monoclonal antibody that defines basal cells of stratified epithelia in various human and rabbit tissues. Histochemistry 92, 319–324 (1989). https://doi.org/10.1007/BF00500547
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DOI: https://doi.org/10.1007/BF00500547