Abstract
The AAL toxins and the fumonisins (FB1 and FB2) are structurally related and produced respectively by Alternaria alternata f.sp. lycopersici and Fusarium moniliforme. AAL toxin is characterized as a hostspecific toxin, toxic to tomato, whereas fumonisin B1 causes equine leukoencephalomalacia. FB1 and FB2 were biologically active in susceptible tomato tissue (Earlypak-7) and animal tissue culture (rat hepatoma H4TG and dog kidney MDCK). Conversely, AAL toxin was also active in the rat and dog tissue culture cells. Both fungi produce toxin/s in culture that cause death in rats; these toxins are other than AAL and fumonisin. The peracetylated derivatives of AAL and FB1 are biologically inactive in both the tomato bioassay and the animal tissue culture systems. Acetylation of the amine renders AAL inactive. The hydrolysis product of AAL (pentolamine) is toxic to the susceptible tomato line whereas the pentolamine of fumonisin is not.
AAL and FB1 can be analyzed by Continuous Flow Fast Atom Bombardment (CFFAB) and Ionspray Mass Spectrometry (ISM), both sensitive to the picomole range. The N-acetyl of the TFA hydrolysis product of AAL and FB1 is determined by comparing the fragment ions at m/z 86 and 140 for FB1 and 72 and 126 for AAL.
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References
Gaumann E. Toxins and plant diseases. Endeavour 1954; 13: 198–204.
Scheffer RP. Host-specific toxins in phytopathology: Origins and evaluation of the concept. In: Kohmoto K, RD Durbin (Eds.). Host-specific toxins. Tottori Japan: Tottori University, 1989.
Scheffer RP. Respiratory effects of the selective toxins of Helminthosporium victoriae. Phytopathology 1963; 53: 465–8.
Scheffer RP, Livingston RS. Host-selective toxins and their role in plant diseases. Science 1984; 223: 17–21.
Tanaka S. Studies on black spot disease of Japanese pears (Pirus serotina Rehd). Mem Coll Agr Kyoto Imp Univ 1933; 28: 1–31.
Grogan RG, Kimble KA, Misaghi I, A stem canker disease of tomato caused by Alternaria alternata f. sp. lycopersici. Phytopathology 1975; 65: 880–86.
Gilchrist DG, Grogan RG. Production and nature of a host-specific toxin from Alternaria alternata f. sp. lycopersici. Phytopathology 1976; 66: 165–171.
Gilchrist DG, Molecular modes of action. In: Daly JM, BJ Deverall (Eds.), Toxins and plant pathogenesis. Academic Press, Sydney: 1983.
Clouse SD, Gilchrist DG. Interaction of the ASC locus in F8 paired lines of tomato with Alternaria alternata f. sp. lycopersici and AAL-toxin. Phytopathology 1987; 77: 80–82.
Bottini AT, Bowen JR, Gilchrist DG. Phytotoxins, II: Characterization of a phyto-toxic fraction from Alternaria alternata f. sp. lycopersici. Tetrahedron Letters 1981; 22: 2723–6.
Schwarte LH, Biester HE, Murray CA. Disease of horses caused by feeding moldy corn. JAVMA 1937; 90: 76–85.
Marasas WFO, Kellerman TS, Pienaar JG, Naude, TW. Leukoencephalomalacia: A mycotoxicosis of equidae caused by Fusarium moniliforme Sheldon. Onderstepoort J Vet Res 1976; 43: 113–22.
Bezuidenhout SC, Gelderblom WCA, Gorst-Almam CP, Horak RM, Marasas WFO, Spiteller G, Veggaar R. Structure elucidation of the fumonisins, mycotoxins from Fusarium moniliforme. J Chem Soc, Chem Commun 1988; 743–45.
Mirocha CJ, Gilchrist DG, Martensen A, Abbas HK, Plasencia J, Vesonder RF. Production of toxins with equivalent host-specific activity, fumonisin and AAL, by monopathogens of tomato. Phytopathology [Abstract]. Paper A370, APS/CPS Annual Meeting, 1990.
Korfmacher WA, Bloom J, Chiarelli MP, Lay Jr, JO, Holcomb M, McManus KT. Comparison of thermospray, FAB, and electrospray for the characterization of the mycotoxin fumonisin B1. Proc 39th ASMS Conference on Mass Spectrometry and Allied Topics (Nashville), 1991.
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Published as paper No. 19,598 of the contribution series of the Minnesota Agricultural Experiment Station based on research conducted under Project 22–34H, supported by HATCH funds.
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Mirocha, C.J., Gilchrist, D.G., Shier, W.T. et al. AAL Toxins, funionisms (biology and chemistry) and host-specificity concepts. Mycopathologia 117, 47–56 (1992). https://doi.org/10.1007/BF00497278
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DOI: https://doi.org/10.1007/BF00497278