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Conjugation of lectins with fluorochromes: An approach to histochemical double labeling of carbohydrate components

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Methodical investigations on the coupling of lectins (Con A, LcL, WGA, RcA) to tetramethylrhodamine isothiocyanate (TRITC) are reported. 20 μg of TRITC per mg of lectin were found to be the optimal amount of TRITC for the conjugation. With this fluorochrome: protein ratio conjugates were produced which resulted in a specific and brilliant fluorescence in tissue staining. The optimally conjugated lectins were separated on DEAE-Sephadex-A 50. Using two different lectins which were conjugated with TRITC or FITC, respectively, a double labeling of different lectin-binding sites in tissue sections was achieved.

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Authors and Affiliations

  1. Institute of Pathology, Friedrich-Schiller University, Ziegelmühlenweg 1, DDR-69, Jena, German Democratic Republic

    Jürgen Roth & Uwe Jens Gerhard

  2. Institute for Cancer Research, University of Vienna, Borschkegasse 8a, A-1090, Vienna, Austria

    Maximillian Binder

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  1. Jürgen Roth
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  2. Maximillian Binder
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  3. Uwe Jens Gerhard
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Roth, J., Binder, M. & Gerhard, U.J. Conjugation of lectins with fluorochromes: An approach to histochemical double labeling of carbohydrate components. Histochemistry 56, 265–273 (1978). https://doi.org/10.1007/BF00495988

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  • DOI: https://doi.org/10.1007/BF00495988

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