Summary
The distribution and cellular localization of leu-enkephalin in the gut and pancreas was studied by immunohistochemistry using two different antisera, one specifically directed against leu-enkephalin and the other cross reacting with met-enkephalin. The results were identical with both antisera. In all species examined, enkephalin-immunoreactive material was found in nerves of the smooth muscle, particularly numerous in the myenteric plexus. Here, immunoreactive nerve cell bodies were observed occasionally. In addition, enkephalin-immunoreactive material was demonstrated in gut endocrine cells of chicken, mouse, rat, pig and monkey but not of guinea pig, cat and man. Enkephalin cells were detected also in the exocrine parenchyma of the porcine pancreas. They were rare in the gut of mouse, rat and monkey but numerous in the antrum and duodenum of pig where they were identified as 5-hydroxytryptamine-storing enterochromaffin cells. The enkephalin-containing cells of the porcine antrum and duodenum were defined ultrastructurally by the consecutive semithin/ultrathin section technique. The ultrastructural features were typical of enterochromaffin cells, the most characteristic ones being the irregular shape and high electron density of the cytoplasmic granules. The immunoreactive material was confined to the cytoplasmic granules.
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Beauvillain, J.C., Tramu, G., Dubois, M.P.: Characterization by different techniques of adrenocorticotropin and gonadotropin producing cells in Lerot pituitary (Eliomys quercinus) Cell Tissue. Res. 158, 301–317 (1975)
Björklund, A., Falck, B., Owman, Ch.: Fluorescence microscopic and microspectrofluorometric techniques for the cellular localization and characterization of biogenic monoamines. In: Methods of investigative and Diagnostic Endocrinology, ed. Berson, S.A., vol. I. The Thyroid and Biogenic Amines (ed. Rall, J.E. and Kopin, I.J.) pp. 318–363. Amsterdam: North Holland 1972
Bloom, F., Battenberg, E., Rossier, J., Ling, N., Leppaluoto, J., Vargo, T.M., Guillemin, R.: Endorphins are located in the intermediate and anterior lobes of the pituitary gland, not in the neurohypophysis. Life Sci. 20, 43–48 (1977)
Creese, I., Snyder, S.H.: Receptor binding and pharmacological activity of opiates in the guinea-pig intestine. J. Pharmacol. Exp. Ther. 194, 205–219 (1975)
Elde, R., Hökfelt, T., Johansson, O., Terenius, L.: Immunohistochemical studies using antibodies to leucine-enkephalin: initial observations on the nervous system of the rat. Neuroscience 1, 349–351 (1976)
Furth, J., Chrétien, M., Lis, M., Belanger, A., Moy, P., Grauman, J.: Multipotent lipotrophic hormones. Arch. Pathol. 99, 572–582 (1975)
Goldstein, A.: Opioid peptides (endorphins) in pituitary and brain. Science 193, 1081–1086 (1976)
Hökfelt, T., Elde, R., Johansson, O., Terenius, L., Stein, L.: The distribution of enkephalin-immunoreactive cell bodies in the rat central nervous system. Neuroscience Lett. 5, 25–31 (1977)
Hughes, J., Smith, T.W., Kosterlitz, H.W., Fothergill, L.A., Morgan, B.A., Morris, H.R.: Identification of two related pentapeptides from the brain with potent opiate against activity. Nature (Lond) 258, 577–579 (1975)
Kosterlitz, H.W., Lord, J.A.H., Watt, A.J.: Morphine receptor in the myenteric plexus of the guinea-pig ileum. In: Agonist and Antagonist Actions of Narcotic Analgesic Drugs (ed. H.W. Kosterlitz, H.O.S. Collier and J.E. Villarreal), pp. 45–61. Baltimore: University Park Press 1973
Lazarus, L.H., Ling, N., Guillemin, R.: β-Lipotropin as a prohormone for the morphinomimetic peptides endorphins and enkephalins. Proc. Natl. Acad. Sci. USA 73, 2156–2159 (1977)
Mayor, H.D., Hampton, J.C., Rosario, R.: A simple method for removing the resin from epoxyembedded tissue. J. biophys. Cytol. 9, 909–910 (1961)
Miller, R.J., Chang, K.-J., Cooper, B., Cuatrecasas, P.: Radioimmunoassay and characterization of enkephalins in rat tissues. J. Biol. Chem. 253, 531–538 (1978)
Moon, H.D., Li, C.H., Jennings, B.M.: Immunohistochemical studies of pituitary β-lipotrophs. Anat. Rec. 175, 529–538 (1973)
Nilsson, G., Larsson, L.-I., Håkanson, R., Brodin, E., Pernow, B., Sundler, F.: Localization of substance P-like immunoreactivity in mouse gut. Histochemistry 43, 97–99 (1975)
Pearse, A.G.E., Polak, J.M.: Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry. Histochem. J. 7, 179–186 (1975)
Pelletier, G., Leclerc, R., Labrie, F., Cote, J., Chretien, M., Lis, M.: Immunohistochemical localization of β-lipotropic hormone in the pituitary gland. Endocrinology 100, 770–776 (1977)
Polak, J., Sullivan, S.N., Bloom, S.R., Facer, P., Pearse, A.G.E.: Enkephalin-like immunoreactivity in the human gastrointestinal tract. Lancet 1977/I, 972–974
Puig, M.M., Gascon, P., Craviso, G.L., Musacchio, J.: Endogenous opiate receptor ligand: Electrically induced release in the guinea pig ileum. Science 195, 419–420 (1977)
Rossier, J., Vargo, T.M., Minick, S., Ling, N., Bloom, F.E., Guillemin, R.: Regional dissociation of β-endorphin and enkephalin contents in rat brain and pituitary. Proc. Natl. Acad. Sci. USA 74, 5162–5165 (1977)
Schulz, R., Wüster, M., Simantov, R., Snyder, S., Herz, A.: Electrically stimulated release of opiate-like material from the myenteric plexus of the guinea pig ileum. Europ. J. Pharmacol. 41, 347–348 (1977)
Simantov, R., Kuhar, M.J., Uhl, G.R., Snyder, S.H.: Opioid peptide enkephalin: Immunohistochemical mapping in rat central nervous system. Proc. Natl. Acad. Sci. USA 74, 2167–2177 (1977)
Sternberger, L.: Immunocytochemistry. Englewood Cliffs, N.J.: Prentice-Hall, Inc. 1974
Sundler, F., Håkanson, R., Larsson, L.-I., Brodin, E., Nilsson, G.: Substance P in the gut: An immunohistochemical and immunohistochemical study of its distribution and development. In: Substance P (eds.: U.S. von Euler and B. Pernow) pp. 59–65. New York: Raven Press 1977
Watson, S.H., Barchas, J.D., Li, C.H.: β-Lipotropin: Localization of cells and axons in rat brain by immunocytochemistry. Proc. Natl. Acad. Sci. USA 74, 5155–5158 (1977)
Yang, H.-Y., Hong, J.S., Costa, E.: Regional distribution of leu and met enkephalin in rat brain. Neuropharmacol. 16, 303–307 (1977)
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Alumets, J., Håkanson, R., Sundler, F. et al. Leu-enkephalin-like material in nerves and enterochromaffin cells in the gut. Histochemistry 56, 187–196 (1978). https://doi.org/10.1007/BF00495979
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DOI: https://doi.org/10.1007/BF00495979