Summary
Dorsal root ganglia L4, L5 were removed to accomplish long-term (1 year) de-afferentation of the rat soleus muscle. Muscle spindles in the muscles deprived of sensory innervation were morphologically and histochemically abnormal. The spindle periaxial fluid space was greatly diminished with a thicker capsular investment. De-afferented intrafusal muscle fibers lacked either “nuclear bags” or “nuclear chains” at their midlengths. The intracapsular myofibrillar ATPase staining pattern of de-afferented nuclear bag fibers resembled that which the bag fibers normally display in their extracapsular regions. These abnormalities are discussed with respect to the regulatory functions of spindle sensory and motor nerves.
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References
Andrew BL, Leslie GC, Part NJ (1978) Some observations on the efferent innervation of rat soleus muscle spindles. Exp Brain Res 31:433–443
Banks RW, Harker DW, Stacey MJ (1977) A study of mammalian intrafusal muscle fibers using a combined histochemical and ultrastructural technique. J Anat 123:783–796
Barker D (1974) The morphology of muscle receptors. In: Hunt CC (ed) Muscle receptors. Springer, Berlin Heidelberg New York (Handbook of sensory physiology, vol III/2, pp 1–190)
Berland D, Chu J, Jones LB, Kaliszewsik J, Lawler W, Oakley B (1977) Physiological evidence for trophic effects: IX the nerve section causes rapid failure of taste responses. Neurosciences (Abstr) 3:533
Brown MC, Holland RL, Ironton R (1978) Degenerating nerve products affect innervated muscle fibers. Nature 275:652–654
Brzezinski DK von (1961) Untersuchungen zur Histochemie der Muskelspindeln. II. Mitteilung: Zur Topochemie und Funktion des Spindelraumes und der Spindelkapsel. Acta Histochem (Jena) 12:277–288
Dubowitz V, Brooke MH (1973) Histological and histochemical stains and reactions. In: Muscle biopsy. W.B. Saunders Co. London, pp 20–33
Guth L, Samaha FJ (1970) Procedure for the histochemical demonstration of actomyosin ATPase. Exp Neurol 28:365–367
Hannerz J, Grimby L (1979) The afferent influence on the voluntary firing range of individual motor units in man. Muscle Nerve 2:414–422
Kucera J (1977a) Histochemistry of intrafusal muscle fibers outside the spindle capsule. Am J Anat 148:427–432
Kucera J (1977b) Intrafusal muscle fiber histochemistry following its motor reinnervation. J Histochem Cytochem 25:1260–1263
Kucera J, Dorovini-Zis K, Engel WK (1978) Histochemistry of rat intrafusal muscle fibers and their motor innervation. J Histochem Cytochem 26:973–988
Kugelberg E (1976) Adaptive transformation of rat soleus motor units during growth. J Neurol Sci 27:269–289
Ovalle WK (1972) Motor nerve terminals on rat intrafusal muscle fibers; a correlated light and electron microscopic study. J Anat 111:239–253
Maynard JA, Tipton CM (1971) The effects of exercise training and denervation on the morphology of intrafusal muscle fibers. Int Z Angew Physiol 30:1–9
Mott FW, Sherrington CS (1895) Experiments upon the influence of sensory nerves upon movement and nutrition of limbs. Proc R Soc Lond 57:481–488
Ranson SW (1928) The role of the dorsal roots in muscle tonus. Arch Neurol Psychiat 19:201–241
Shantha TR, Golarz MN, Bourne GH (1968) Histological and histochemical observations on the capsule of the muscle spindle in normal and denervated muscle. Acta Anat. (Basel) 69:632–646
Tower SS (1932) Atrophy and degeration in the muscle spindle. Brain 55:77–90
Yellin H (1974) Regional differences in the contractile apparatus of intrafusal muscle fibers. Am J Anat 139:147–152
Vrbova G (1978) The influence of the motor nerve on the characteristic properties of skeletal muscle. In: Lunt GG, Marchbanks RM (eds) The biochemistry of myasthenia gravis and musclar dystrophy. Academic Press, London, pp 3–21
Zelena J (1964) Development, degeneration and regeneration of receptor organs. Prog Brain Res 13:206–211
Zelena J, Soukup T (1974) The differentiation of intrafusal fibre types in rat muscle spindles after motor denervation. Cell Tissue Res 153:115–136
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Kucera, J. Myofibrillar ATPase activity of intrafusal fibers in chronically de-afferented rat muscle spindles. Histochemistry 66, 221–228 (1980). https://doi.org/10.1007/BF00494648
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DOI: https://doi.org/10.1007/BF00494648