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Histochemistry of glycoconjugates in the gallbladder epithelium of ten animal species

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Summary

A battery of seven lectins and several conventional mucin histochemical techniques were used to identify the epithelial mucins of the gallbladder of ten species: man, rabbit (Oryctolagus cuniculus, mammalia), hamster (Mesocricetus auratus, mammalia), chicken (Gallus gallus, bird), sparrow (Passer domesticus, bird), moorish gecko (Tarentola mauritanica, reptilia), ladder snake (Elaphe scalaris, reptilia), lake frog (Rana perezi, amphibia), natterjack toad (Bufo calamita, amphibia) and gilthead sea bream (Sparus auratus, fish). Glycogen was found in the epithelial lining of the reptilian and amphibian gallbladders. Sulphate and carboxyl groups were frequently found in the same species, except in the ladder snake and natterjack toad gallbladders where only sulphate groups were identified. Sialic acid residues were detected in man, rabbit, bird, T. mauritanica, R. perezi and fish gallbladders. ConA binding pattern was similar in the ten species studied. In the human gallbladder only PNA failed to label the luminal surface, while the glands were only unreactive to DBA. The human gallbladder showed a large variety of saccharides. The present results suggest that no relation exists between the composition of the gallbladder mucins and the situation of the species in the phylogenetic scale.

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References

  • Bhavanandan VP, Katlic AW (1979) The interaction of wheat germ agglutinin with sialoglycoproteins. The role of sialic acid. J Biol Chem 254:4000–4008

    Google Scholar 

  • Culling CFA, Reid PE, Dunn WL, Clay MG (1974) The histochemical demonstration of O-acylated sialic acids in gastrointestinal mucins. Their association with the potassium hydroxide-periodic acid-Schiff effect. J Histochem Cytochem 22:826–831

    Google Scholar 

  • Debray H, Decout D, Strecker G, Spik G, Montreuil J (1981) Specifity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosylproteins. Eur J Biochem 117:41–55

    Google Scholar 

  • Ekblom P, Miettinen M, Rapola I, Foidart JM (1982) Demonstration of laminin, a basement membrane glycoprotein in routinely processed formalin fixed human tissues. Histochemistry 75:301–307

    Google Scholar 

  • Gabe M (1968) Techniques histologiques. Masson, Paris

    Google Scholar 

  • Ganter P, Jolles G (1969) Histochimie normale et pathologique. Gauthier-Villars, Paris

    Google Scholar 

  • Geleff S, Böck P (1984) Lectin binding of surface epithelia and concomitant glands of gallbladder and biliary ducts in the guinea pig. Histochemistry 81:543–549

    Google Scholar 

  • Gheri G, Gheri Bryck S, Orlandini GE (1988) Histochemistry of mucosubstances in the gallbladder epithelium of the chick embryo. Histochemistry 88:519–524

    Google Scholar 

  • Giles RB Jr, Smith JE, Crowley G, Michael M (1960) A study of carbohydrates in human gall bladder bile. J Lab Clin Med 55:38–45

    Google Scholar 

  • Goldstein IJ, Hayes CE (1978) The lectins: carbohydrate-binding proteins of plants and animals. Adv Carbohydr Chem Biochem 35:127–340

    Google Scholar 

  • Goldstein IJ, Hammarström S, Sundblad G (1975) Precipitation and carbohydrate-binding specifity studies on wheat-germ agglutinin. Biochim Biophys Acta 405:53–61

    Google Scholar 

  • Grzycki S, Rzeszowska G (1969) Glycogen and acid mucopolysaccharides in the epithelium of gallbladder mucosa under nonexperimental conditions. Z Mikrosk Anat Forsch 79:477–484

    Google Scholar 

  • Häkkinen I, Laitio M (1970) Epithelial glycoproteins of human gallbladder. Immunological characterization. Arch Pathol 90:137–142

    Google Scholar 

  • Karayannopoulou G, Damjanov I (1987) Lectin binding sites in the human gallbladder and cystic duct. Histochemistry 88:75–83

    Google Scholar 

  • Laitio M (1980) Morphology and histochemistry of non-tumorous gallbladder epithelium. A series of 103 cases. Pathol Res Pract 167:335–345

    Google Scholar 

  • LaMont JT, Smith BF, Moore JRL (1984) Role of gallbladder mucin in pathophysiology of gallstones. Hepatology 4:51S-56S

    Google Scholar 

  • Leathem AJC, Atkins J (1983) Lectin binding to paraffin sections. In: Bullock GR, Petrusz P (eds) Techniques in immunocytochemistry, vol 2. Academic Press, London, pp 39–70

    Google Scholar 

  • Lis H, Sela BA, Sachs L, Sharon N (1970) Specific inhibition by N-acetyl-D-galactosamine of the interaction between soybean agglutinin and animal cell surfaces. Biochim Biophys Acta 211:582–585

    Google Scholar 

  • Lopes RA, Dos Santos HSL, Petenusci SO, Miglino MA, de Souza WM, Boccaletti D (1983) The gallbladder of the Capivara Hydrochoerus hydrochoeris (Mammalia: Rodentia). Gegenbaurs Morphol Jahrb (Leipzig) 129:483–488

    Google Scholar 

  • Lotan R, Skutelsky E, Danon D, Sharon N (1975) The purification, composition, and specifity of the anti T-lectin from peanut (Arachis hipogaca). J Biol Chem 250:8518–8523

    Google Scholar 

  • Luciano L, Reale E, Wolpers C (1974) Die Feinstruktur der Gallenblase und der Gallenlänge. V. Histochemische Lokalisierung von Mukosubstanzen im menschlichen Gallenblasenepithel. Histochemistry 38:57–70

    Google Scholar 

  • Maki T, Matsushiro T, Suzuki N, Nakamura N (1971) Role of sulfated glycoprotein in gallstone formation. Gynecol Obstet 132:846–854

    Google Scholar 

  • Martoja R, Martoia M (1970) Técnicas de histologia animal. Toray-Masson, Barcelona

    Google Scholar 

  • Matsumoto I, Osawa T (1969) Purification and characterization of an anti-H(O) phytohemagglutinin of Ulex europaeus. Biochim Biophys Acta 194:180–189

    Google Scholar 

  • MacManus JFA (1946) Histological and histochemical uses of periodic acid. Stain Technol 23:99–108

    Google Scholar 

  • Neiderhiser DH, Plantner JJ, Carlson DM (1971) The purification and properties of the glycoproteins of pig gallbladder bile. Arch Biochem Biophys 145:155–163

    Google Scholar 

  • Pearse AGE (1985) Histochemistry, Theoretical and applied, vol 2. Churchill Livingstone, London

    Google Scholar 

  • Pearson JP, Kaura R, Taylor W, Allen A (1982) The composition and polymeric structure of mucus glycoprotein from human gallbladder bile. Biochim Biophys Acta 706:221–228

    Google Scholar 

  • Pereira MEA, Kabat EA (1974) Blood group specifity of the lectin Lotus tetragonolobus. Ann NY Acad Sci 234:301–305

    Google Scholar 

  • Reid PE, Culling CFA, Dunn WL (1973) Saponification induced increase in the periodic acid-Schiff reaction in the gastrointestinal tract: mechanism and distribution of the reactive substance. J Histochem Cytochem 21:473–482

    Google Scholar 

  • Roberts GP (1977) Histochemical detection of sialic acid residues using periodate oxidation. Histochem J 9:97–102

    Google Scholar 

  • Schiebler TH, Lang K, Wahlin T (1975) Zur Entwicklung des Gallenblasenepithels des Meerschweinchens. I. Färberisch-lichtmikroskopische und kohlenhydrat-histochemische Untersuchunger. Histochemistry 45:177–190

    Google Scholar 

  • Spicer SS (1965) Diamine methods for differentiating mucosubstances histochemically. J Histochem Cytochem 13:211–234

    Google Scholar 

  • Togari CH, Okada T (1954) Cytological observations on the epithelium of the gallbladder in the urodele amphibian (Triturus pyrrhogaster). Okajimas Folia Anat Jpn 26:327–333

    Google Scholar 

  • Togari CH, Okada T (1960) Cytological studies on the gallbladder epithelium of the fish. Okajimas Folia Anat Jpn 35:11–26

    Google Scholar 

  • Turchini J, Catayee G, Perez A (1958) Histochemistry of several compounds in the gall bladder of Rhinolophus hipposideros in hibernation. Ann Histochim 3:39–44

    Google Scholar 

  • Tusques J, Senelar R, Ginguene Y, Vaugien M (1964) Etude histochimique sur les vésicules biliaries de mouton et de cobaye: mucopolysaccharides. Ann Histochim 9:269–274

    Google Scholar 

  • Varute AT, Nalavade MN (1973) Histochemical analysis of mucosubstances in the gall bladder epithelium of the domestic cat. Folia Histochem Cytochem 11:21–32

    Google Scholar 

  • Viehberger G (1983) Ultrastructural and histochemical study of the gallbladder epithelia of rainbow trout and tench. Tissue Cell 121–135

  • Wahlin T (1979) Histochemical analysis of mucosubstances and cytochemical studies of the Golgi region and the secretory granules of the normal gerbil gallbladder principal cells. Acta Anat 103:468–476

    Google Scholar 

  • Wahlin T, Bloom GD, Carlsöö B (1974) Histochemical observations with the light and the electron microscope on the mucosubstances of the normal mouse gallbladder epithelial cells. Histochemistry 42:119–131

    Google Scholar 

  • Yamada K (1960) A unique mode of apocrine secretion in the gall bladder epithelium of Clemmys japonica with a note on the histochemical quality of the secretory material. Okajimas Folia Anat Jpn 36:233–241

    Google Scholar 

  • Yamada K (1962a) Chemocytological observations on two peculiar epithelial cell types in the gall bladders of laboratory rodents. Z Zellforsch 56:180–187

    Google Scholar 

  • Yamada K (1962b) Morphological and histochemical aspects of secretion in the gall bladder epithelium of the guinea pig. Anat Rec 144:117–127

    Google Scholar 

  • Yamada K (1963) A contribution to the histochemistry of acid mucin in the gall bladder epithelium of the toad (Bufo vulgaris japonicus). Acta Histochem 15:50–57

    Google Scholar 

  • Yamada K, Hoshino M (1972) Morphochemical analysis of epithelial mucosubstances in the gall bladder of the fowl (Gallus domesticus). Histochemie 29:120–128

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Cell Biology, Section of Histology and General Embryology, School of Medicine, University of Murcia, E-30100, Murcia, Spain

    J. F. Madrid, J. Ballesta, T. Galera, M. T. Castells & R. Pérez-Tomás

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  1. J. F. Madrid
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  2. J. Ballesta
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  3. T. Galera
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  4. M. T. Castells
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  5. R. Pérez-Tomás
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Madrid, J.F., Ballesta, J., Galera, T. et al. Histochemistry of glycoconjugates in the gallbladder epithelium of ten animal species. Histochemistry 91, 437–443 (1989). https://doi.org/10.1007/BF00493832

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