Abstract
Neurospora crassa can utilize glucose 6-sulfate as its sole sulfur source, although this compound cannot serve as a carbon source for this organism. Neurospora possesses a transport system capable of glucose 6-sulfate uptake; the system is energy dependent, is inhibited by extracellular sulfate, and is clearly distinct from the permeases responsible for the uptake of glucose and those for sulfate transport. The metabolism of glucose 6-sulfate apparently involves its transport as an intact molecule, followed by a slow intracellular hydrolysis. Methionine, which represses the synthesis of a number of enzymes of sulfur anabolism, also represses the synthesis of the transport system responsible for glucose 6-sulfate uptake. A regulatory gene, cys-3, which controls the synthesis of aryl sulfatase, choline sulfatase, choline-O-sulfate permease, and two distinct permease species, also regulates the permease for glucose 6-sulfate.
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This investigation was supported by Public Health Service Grant 5 RO1 GM-18642 from the National Institute of General Medical Services.
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Reinert, W.R., Marzluf, G.A. Regulation of sulfate metabolism in Neurospora crassa: Transport and accumulation of glucose 6-sulfate. Biochem Genet 12, 97–108 (1974). https://doi.org/10.1007/BF00487819
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DOI: https://doi.org/10.1007/BF00487819