Abstract
Arg-2, one of the first arginine-requiring mutants isolated in Chlamydomonas reinhardi, has long been regarded as lacking the enzyme argininosuccinate synthetase. In view of various discrepancies found in the literature, the position of this mutant has been reviewed. The results show that arg-2 has a normal argininosuccinate synthetase activity but lacks argininosuccinate lyase. This finding is in agreement with the results of recombination and complementation analysis, which indicate that arg-2 is included in the arg-7 cistron.
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References
Archibald, R. N. (1944). Determination of citrulline and allantoin and demonstration of citrulline in blood plasma. J. Biol. Chem. 156121.
Ebersold, W. T. (1956). Crossing-over in Chlamydomonas reinhardi. Am. J. Bot. 43408.
Ebersold, W. T. (1967). Chlamydomonas reinhardi: Heterozygous diploid strains. Science 157447.
Eversole, R. A. (1956). Biochemical mutants of Chlamydomonas reinhardi. Am. J. Bot. 43404.
Fincham, J. R. S. (1959). On the nature of the glutamic dehydrogenase produced by interallelic complementation at the am locus of Neurospora crassa. J. Gen. Microbiol. 21600.
Fincham, J. R. S. (1966). Genetic Complementation, W. A. Benjamin, New York.
Garen, A., and Garen, S. (1963). Complementation in vivo between structural mutants of alkaline phosphatase from E. coli. J. Mol. Biol. 713.
Gillham, N. W. (1965). Induction of chromosomal and nonchromosomal mutations in Chlamydomonas reinhardi with N-methyl-N'-nitro-N-nitrosoguanidine. Genetics 52529.
Gross, S. R., and Webster, R. E. (1963). Some aspects of interallelic complementation involving leucine biosynthetic enzymes of Neurospora. Cold Spring Harbor Symp. Quant. Biol. 28543.
Hirs, C. H. W., Moore, S., and Stein, W. M. (1954). The chromatography of amino acids on ion exchange resins. Use of volatile acids for elution. J. Am. Chem. Soc. 766063.
Hudock, G. A. (1962). The pathway of arginine biosynthesis in Chlamydomonas reinhardi. Biochem. Biophys. Res. Commun. 9551.
Hudock, G. A. (1963). Repression of argininosuccinase in Chlamydomonas reinhardi. Biochem. Biophys. Res. Commun. 10133.
Levine, R. P., and Ebersold, W. T. (1958). Gene recombination in Chlamydomonas reinhardi. Cold Spring Harbor Symp. Quant. Biol. 23101.
Levine, R. P., and Goodenough, U. W. (1970). The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi. Ann. Rev. Genet. 4397.
Loppes, R. (1969). A new class of arginine-requiring mutants in Chlamydomonas reinhardi. Molec. Gen. Genet. 104172.
Loppes, R. (1970). Selection of arginine-requiring mutants after treatment with three mutagens. Experientid (Basel) 26660.
Loppes, R., and Strijkert, P. J. (1972). Arginine metabolism in Chlamydomonas reinhardi. Conditional expression of arginine-requiring mutants. Molec. Gen. Genet. 116298.
Loppes, R., Matagne, R., and Strijkert, P. J. (1972). Complementation at the Arg-7 Locus in Chlamydomonas reinhardi. Heredity 28239.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193265.
Ratner, S. (1955). Enzymatic synthesis of arginine (condensing and splitting enzymes). In Methods in Enzymology, Vol. II, Academic Press, New York, p. 356.
Ratner, S., and Kunkemueller, M. (1966). Separation and properties of argininosuccinate and its two anhydrides, and their detection in biological materials. Biochemistry 51821.
Reger, B. J., Fairfield, S. A., Epler, J. L., and Barnett, W. E. (1970). Identification and origin of some chloroplast aminoacyl-tRNA synthetases and tRNAs. Proc. Natl. Acad. Sci. 671207.
Strijkert, P. J., and Sussenbach, J. S. (1969). Arginine metabolism in Chlamydomonas reinhardi. Evidence for a specific regulatory mechanism of the biosynthesis. Europ. J. Biochem. 8408.
Sussenbach, J. S., and Strijkert, P. J. (1969a). Arginine metabolism in Chlamydomonas reinhardi. On the regulation of the arginine biosynthesis. Europ. J. Biochem. 8408.
Sussenbach, J. S., and Strijkert, P. J. (1969a). Arginine metabolism in Chlamydomonas reinhardi. On the regulation of the arginine biosynthesis. Europ. J. Biochem. 8403.
Sussenbach, J. S., and Strijkert, P. J. (1969b). Arginine metabolism in Chlamydomonas reinhardi. Arginine deiminase: The first enzyme of the catabolic pathway. FEBS Letters 3166.
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Chercheur qualifié du Fonds National Belge de la Recherche Scientifique.
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Strijkert, P.J., Loppes, R. & Sussenbach, J.S. The actual biochemical block in the arg-2 mutant of Chlamydomonas reinhardi . Biochem Genet 8, 239–248 (1973). https://doi.org/10.1007/BF00486176
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DOI: https://doi.org/10.1007/BF00486176