Abstract
The levels of the carbonic anhydrase isozymes (CA and CA II) in single erythrocytes of DBA/2J mice were assayed by measuring the specific immunofluorescence of CA I and CA II with a microspectrofluorometric technique. Measurements of 100 randomly selected cells showed a range (in relative fluorescence units) of 21–52 (mean 31.3±7.5) for CA I and 30–80 (mean 45.6±10.7) for CA II. The CA II/CA I ratio of the means obtained by the single-cell fluorescence assay was similar to the ratios obtained for the two isozymes from hemolysates of DBA/2J mice using an immunodiffusion assay. The influence of cell age on the variation in carbonic anhydrase levels was determined by separating red cells into several fractions by a gravity sedimentation procedure. The younger cells showed higher levels of CA I and CA II than the older cells; however, the extensive overlap in the variability between the cells from the different fractions indicated that although cell age was contributing to the overall heterogeneity, its influence was not pronounced.
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This investigation was supported by USPHS Grant GM 15419 and Training Grant 5-TO1-GM-71-18 (S. S. H.).
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Hsu, S.S., Tashian, R.E. Genetic control of quantitative variation in carbonic anhydrase isozymes of mammals. I. The concentration within single erythrocytes of the mouse. Biochem Genet 15, 885–896 (1977). https://doi.org/10.1007/BF00483984
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DOI: https://doi.org/10.1007/BF00483984