Summary
Cytochrome P-450, detectable in n-hexadecane-grown cells of Acinetobacter calcoaceticus, is not found during growth on complex media, sugars, or various metabolic indermediates, such as mono- or dicarboxylic acids. Cytochrome P-450 formation is observed after shifting cells from a non-hydrocarbon medium to a minimal medium with n-hexadecane as the sole source of carbon, or after addition of n-hexadecane to cultures growing on a non-inducing carbon source. The content increases with time. Induction of cytochrome P-450 is inhibited by streptomycin, chloramphenicol, and rifampicin. Besides n-hexane, other n-alkanes from n-hexane up to n-hexadecane are inducers, whereas cetyl alcohol or palmitic acid are not. The results indicate that the occurrence of cytochrome P-450 in n-alkane-induced cells reflects a de novo protein synthesis. Regulation seems not to be governed by catabolite repression but by the presence of an inducer molecule, which is either an n-alkane or a very similar molecule.
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Asperger, O., Naumann, A. & Kleber, HP. Inducibility of cytochrome P-450 in Acinetobacter calcoaceticus by n-alkanes. Appl Microbiol Biotechnol 19, 398–403 (1984). https://doi.org/10.1007/BF00454377
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DOI: https://doi.org/10.1007/BF00454377