Abstract
A temperature-sensitive mutant of Neurospora crassa was found to undergo rapid death on minimal medium at 35°C. The loss of viability in this mutant was prevented by various factors which retard growth, including deprivation of carbon sources or interruption of protein synthesis. Synthesis of nucleic acids and protein in this mutant was normal at the early stages of germination and then depressed at 35°C. The active transport of glucose and the respiration rate in this mutant were depressed at 35°C. Phospholipid synthesis was significantly repressed at 35°C. The possible significance of the characteristics of this mutant is discussed in terms of membrane biosynthesis.
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Beadle, G. W., Tatum, E. L.: Neurospora. II. Methods of producing and detecting mutations concerned with nutritional requirement. Amer. J. Bot. 32, 678–686 (1945)
Bray, G. A.: A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analyt. Biochem. 1, 279–285 (1960)
Cronan, J. E., Jr., Ray, T. K., Vagelos, P. R.: Selection and characterization of an E. coli mutant defective in membrane lipid biosynthesis. Proc. nat. Acad. Sci. (Wash.) 65, 737–744 (1970)
Estabrook, R. W.: Mitochondrial respiratory control and the polarographic measurement of ADP: O ratios. In: Methods in enzymology, Vol. 10, R. W. Estabrook, M. E. Pullman, eds., pp. 41–47. New York-London: Academic Press 1967
Folch, J., Lees, M., Stanley, G. H. S.: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. 226, 497–509 (1057)
Hechemy, K., Goldfine, H.: Isolation and characterization of a temperature-sensitive mutant of Escherichia coli with a lesion in the acylation of lysophosphatidic acid. Biochem. biophys. Res. Commun. 42, 245–251 (1971)
Henry, S. A.: Death resulting from fatty acid starvation in yeast. J. Bact. 116, 1293–1303 (1973)
Inoue, H., Ishikawa T.: Macromolecule synthesis and germination of conidia in temperature sensitive mutants of Neurospora crassa. Jap. J. Genet. 45, 357–369 (1970)
Kuraishi H., Takamura, Y., Mizunaga, T., Uemura, T.: Factors influencing death of biotin deficient yeast cells. J. gen. appl. Microbiol. 17, 29–42 (1971)
Lester, H. E., Gross, S. R.: Efficient method for selection of auxotrophic mutants of Neurospora. Science 129, 572 (1959)
Littlewood, B. S.: A method for obtaining antibioticsensitive mutants in Saccharomyces cerevisiae. Genetics 73, 305–308 (1972)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Pontecorvo, G., Roper, J. A., Hemmons, L. M., Mac-Donald, K. D., Bufton, A. W. J.: The genetics of Aspergillus nidulans. Advanc. Genet. 5, 141–238 (1953)
Ridgway, G. J., Douglas, H. C.: Unbalanced growth of yeast due to inositol deficiency. J. Bact. 76, 163–166 (1958)
Ryan, F. J., Beadle, G. W., Tatum, E. L.: The tube method of measuring the growth rate of Neurospora. Amer. J. Bot. 30, 784–799 (1943)
Scarborough, G. A.: Sugar transport in Neurospora crassa. J. biol. Chem. 245, 1695–1698 (1970)
Schmidt, G., Thannhauser, S. J.: A method for the determination of deoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues. J. biol. Chem. 161, 83–89 (1945)
Schneider, R. P., Wiley, W. R.: Kinetic characteristics of the two glucose transport systems in Neurospora crassa. J. Bact. 106, 479–486 (1971)
Shatkin, A. J., Tatum, E. L.: The relationship of m-inositol to morphology in Neurospora crassa Amer. J. Bot. 48, 760–771 (1961)
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Inoue, H., Ishikawa, T. Death resulting from unbalanced growth in a temperature-sensitive mutant of Neurospora crassa . Arch. Microbiol. 104, 1–6 (1975). https://doi.org/10.1007/BF00447292
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DOI: https://doi.org/10.1007/BF00447292