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Antibiotic tetaine — a selective inhibitor of chitin and mannoprotein biosynthesis in Candida albicans

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Abstract

The antibiotic tetaine inhibits in Candida albicans the biosynthesis of two important cell wall constituents, chitin and mannoprotein. This effect is a consequence of inactivation of the enzyme glucosamine-6-phosphate synthetase. Due to the lack of glucosamine-6-phosphate the effective secretion of mannoprotein enzymes, acid phosphatase and invertase, by Candida albicans spheroplasts is inhibited. In the presence of tetaine, probably a modified mannoprotein, lacking a branched polymannan, is synthesized. The antibiotic action decreases the viability of Candida albicans cells, especially that of mycelial forms of this fungus.

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Abbreviations

GlcNAc:

N-acetyl-d-glucosamine

GlcN-6-P:

d-glucosamine-6-phosphate

ManNAc:

N-acetyl-d-mannosamine

α-MM:

α-methylmannoside

EGTA:

1,2 di/2-aminoethoxy/ethane

N,N,N′,N′:

tetra-acetic acid

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Authors and Affiliations

  1. Department of Pharmaceutical Technology and Biochemistry, Technical University, PL-80-952, Gdanśk, Poland

    Slawomir Milewski, Henryk Chmara & Edward Borowski

Authors
  1. Slawomir Milewski
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  2. Henryk Chmara
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  3. Edward Borowski
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Milewski, S., Chmara, H. & Borowski, E. Antibiotic tetaine — a selective inhibitor of chitin and mannoprotein biosynthesis in Candida albicans . Arch. Microbiol. 145, 234–240 (1986). https://doi.org/10.1007/BF00443651

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  • DOI: https://doi.org/10.1007/BF00443651

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