Summary
Chromosomes XII and XIII of a Saccharomyces carlsbergensis brewing strain were analysed after their transfer into Saccharomyces cerevisiae by kar1-mediated single chromosome transfer. The lager yeast was found to be heterozygous for the isoleucine-valine biosynthesis genes IL V2 (encoding acetohydroxy acid synthase) and IL V5 (encoding acetohydroxy acid reductoisomerase). In both cases, Southern analysis showed restriction site polymorphisms, and that one allele hybridizes more strongly to that of S. cerevisiae than the other. The alleles with limited nucleotide sequence homology are located on chromosomes which recombine poorly with the corresponding S. cerevisiae chromosomes (XIII and XII) during meiosis. A cluster of ribosomal RNA genes is located on the chromosome XII with the S. cerevisiae-like IL V5, but not on the homoeologous chromosome. The present analysis supports the view that S. carlsbergensis is an amphiploid hybrid.
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Appels R, Gerlach WL, Dennis ES, Swift H, Peacock WJ (1980) Chromosoma 78:293–311
Bell GI, DeGennaro LJ, Gelfand DH, Bishop RJ, Valenzuela P, Rutter WJ (1977) J Biol Chem 252:8118–8125
Casey GP (1986) Carlsberg Res Commun 51:343–362
Conde J, Fink GR (1976) Proc Natl Acad Sci USA 73:3651–3655
Davis RW, Thomas M, Cameron J, StJohn TP, Scherer S, Padgett RA (1980) Methods Enzymol 65 (part I); 404–4111
Dutcher SK (1981) Mol Cell Biol 1:245–253
Falco SC, Dumas KS (1985) Genetics 109:21–35
Falco SC, Dumas KS, Livak KJ (1985) Nucleic Acids Res 13:4011–4027
Gjermansen C, Sigsgaard P (1981) Carlsberg Res Commun 46:1–11
Holmberg S (1982) Carlsberg Res Commun 47:233–244
Holmberg S, Petersen JGL, Nilsson-Tillgren T, Gjermansen C, Kielland-Brandt MC (1985) Biosynthesis of isoleucine and valine in Saccharomyces. Gene structure and regulation. In: Stewart GG, Russel I, Klein D, Hiebsch RR (eds) Biological research on industrial yeasts, vol I. Uniscience Series, CRC Press, Boca Raton (in press)
Kakar SN, Wagner RP (1964) Genetics 49:213–222
Magee PT, de Robichon-Szulmajster H (1968) Eur J Biochem 3:502–506
Maniatis T, Fritsch EF, Sambrook J (1984) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Mortimer RK, Schild D (1984) Microbiol Sci 1:145–146
Nilsson-Tillgren T, Petersen JGL, Holmberg S, Kielland-Brandt MC (1980) Carlsberg Res Commun 45:113–117'
Nilsson-Tillgren T, Gjermansen C, Kielland-Brandt MC, Petersen JGL, Holmberg S (1981) Carlsberg Res Commun 46:65–76
Nilsson-Tillgren T, Kielland-Brandt MC, Holmberg S, Petersen JGL, Gjermansen C (1982) Proc 4th Int Symp Genet Industr Microorg, Kyoto, pp 143–147
Nilsson-Tillgren T, Gjermansen C, Holmberg S, Petersen JGL, Kielland-Brandt MC (1986) Carlsberg Res Commun 51:309–326
Pedersen MB (1985) Carlsberg Res Commun 50:263–272
Pedersen MB (1986) Carlsberg Res Commun 51:185–202
Petersen JGL, Holmberg S (1986) Nucleic Acids Res 14:9631–9651
Petersen JGL, Kielland-Brandt MC, Holmberg S, Nilsson-Tillgren T (1983a) Carlsberg Res Commun 48:21–34
Petersen JGL, Kielland-Brandt MC, Holmberg S, Nilsson-Tillgren T, Gjermansen C (1983b) Homeologous ILV genes in Saccharomyces. In: Korhola M, Vaisanen E (eds) Gene expression in yeast. Foundation for Biotechnical and Industrial Fermentation Research, Helsinki, Finland, pp 201–207
Petes TD (1979) J Bacteriol 138:185–192
Petes TD, Hereford LM, Skryabin KG (1978) J Bacteriol 134:295–305
Polaina J (1984) Carlsberg Res Commun 49:577–584
Rothstein RJ (1983) Methods Enzymol 101:202–211
Schweizer E, MacKechnie C, Halvorson HO (1969) J Mol Biol 40:261–277
Sherman F, Fink GR, Lawrence CW (1974) Laboratory manual for a course, Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Spencer JFT, Spencer DM (1983) Annu Rev Microbiol 37:121–142
Wainwright T (1973) J Inst Brew 79:451–470
Winston F, Chumley F, Fink GR (1983) Methods Enzymol 101:211–228
Wood JS (1982) Mol Cell Biol 2:1080–1087
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Petersen, J.G.L., Nilsson-Tillgren, T., Kielland-Brandt, M.C. et al. Structural heterozygosis at genes IL V2 and IL V5 in Saccharomyces carlsbergensiss . Curr Genet 12, 167–174 (1987). https://doi.org/10.1007/BF00436875
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DOI: https://doi.org/10.1007/BF00436875