Abstract
The lithium pump in intact human erythrocytes which has previously been shown to be repressed following lithium carbonate ingestion by manicdepressive subjects is now shown to be inhibited selectively by N-ethylmaleimide and ruthenium red, both of which are known to inhibit the erythrocyte Ca pump. It is proposed that active lithium efflux is linked to the concurrent operation of the calcium pump.
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Bond, G. H.: Ligand-induced conformational changes in the (Mg2++Ca2+)-dependent ATPase of red cell membranes. Biochim. Biophys. Acta 288, 423–433 (1972)
Cha, Y. N., Shin, B. C., Lee, K. S.: Active uptake of Ca2+ and Ca2+ activated Mg2+-ATPase in red cell fragments. J. Gen. Physiol. 57, 202–215 (1971)
Duhm, J., Eisenried, F., Becker, B. F., Greil, W.: Studies on the lithium transport across the red cell membrane. Li+ uphill transport by the Na+-dependent Li+ counter-transport system of human erythrocytes. Pflügers Arch. 364, 147–155 (1976)
Dunham, E. T., Glynn, I. M.: Adenosinetriphosphatase activity and the active movement of alkali cations. J. Physiol. (Lond.) 156, 274–293 (1961)
Dunn, M. J.: Red blood cell calcium and magnesium effects upon sodium and potassium transport and cellular morphology. Biochim. Biophys. Acta 353, 97–116 (1974)
ferreira, H. G., Lew, V. L.: Use of ionophore A23187 to measure cytoplasmic Ca buffering and activation of the Ca pump by internal Ca. Nature 259, 47–49 (1976)
Haas, M., Schooler, J., Tosteson, D. C.: Coupling of lithium to sodium transport in human red cells. Nature 258, 425–427 (1975)
Jacob, H. S., Jandl, J. H.: Effects of sulfhydryl inhibition on red blood cells. Mechanism of hemolysis. J. Clin. Invest. 41, 779–792 (1961)
Knauf, P. A., Proverbio, F., Hoffman, J. F.: Electrophoretic separation of different phosphoproteins associated with Ca-ATPase and Na,K-ATPase in human red cell ghosts. J. Gen. Physiol. 63, 324–336 (1974)
Lee, K. S., Shin, B. C.: Studies on the active transport of calcium in human red cells. J. Gen. Physiol. 54, 713–728 (1969)
Luft, J. H.: Ruthenium red and violet. Chemistry, purification, methods of electron microscopy and mechanism of action. Anat. Rec. 171, 347–368 (1971a)
Luft, J. H.: Ruthenium red and violet. Fine structural localization in animal tissue. Anat. Rec. 171, 369–416 (1971b)
Meltzer, H. L., Rosoff, C. J., Kassir, S., Fieve, R. R.: Active efflux of lithium from erythrocytes of manic-depressive subjects. Life Sci. 19, 371–380 (1976)
Meltzer, H. L., Kassir, S., Dunner, D. L., Fieve, R. R.: Repression of a lithium pump as a consequence of lithium ingestion by manicdepressive subjects. Psychopharmacology 54, 113–118 (1977)
Olson, E. J., Cazort, R. J.: Active calcium and strontium transport in human erythrocyte ghosts. J. Gen. Physiol. 53, 311–322 (1969)
Pandey, G. N., Javaid, J. I., Davis, J. M., Tosteson, D. C.: Mechanism of lithium transport in red blood cells. Physiologist 19, 321 (1976)
Porzig, H.: Calcium efflux from human erythrocyte ghosts. J. Membr. Biol. 2, 324–339 (1970)
Quist, E. E., Roufogalis, B. D.: Determination of the stoichiometry of the calcium pump in human erythrocytes using lanthanum as a selective inhibitor. FEBS Lett. 50, 135–139 (1975)
Rafaelsen, O. J., Mellerup, E. T., Shapiro, R. W.: Lithium in the living organism. Is an integrated hypothesis possible? Pharmacopsychology 9, 108–115 (1976)
Richards, D. E., Rega, A. F., Garrahan, P. J.: ATPase and phosphatase activities from human red cell membranes. J. Membr. Biol. 35, 113–124 (1977)
Romero, P. J., Whittam, R.: The control by internal calcium of membrane permeability to sodium and potassium. J. Physiol. (Lond.) 214, 481–507 (1971)
Sarkadi, B., Szasz, I., Gerloczy, A., Gardos, G.: Transport parameters and stoichiometry of active calcium ion extrusion in intact human red cells. Biochim. Biophys. Acta 464, 93–107 (1977)
Schatzmann, H. J.: ATP-dependent Ca2+ extrusion from human red cells. Experientia 22, 364–368 (1966)
Schatzmann, H. J.: Dependence on calcium concentration and stoichiometry of the calcium pump in human red cells. J. Physiol. (Lond.) 235, 551–569 (1973)
Schatzmann, H. J., Vincenzi, F. F.: Calcium movements across the membrane of human red cells. J. Physiol. (Lond.) 201, 369–395 (1969)
Schrier, S. L., Bensch, K. G., Johnson, M., Junga, I.: Energized endocytosis in human erythroxyte ghosts. J. Clin. Invest. 56, 8–22 (1975)
Shapiro, B., Kollmann, G., Asnen, I.: Mechanism of the effect of ionizing radiation on sodium uptake by human erythrocytes. Radiation Res. 27, 139–158 (1966)
Smith, R. P. P., Ellman, G. L.: A study of the dependence of the human erythrocyte glucose transport system on membrane sulfhydryl groups. J. Membr. Biol. 12, 177–188 (1973)
Vincenzi, F. F., Schatzmann, H. J.: Some properties of Ca-activated ATPase in human red cell membranes. Helv. Physiol. Pharmacol. Acta 25, 233–234 (1967)
Watson, E. L., Vincenzi, F. F., Davis, P. W.: Ca2+-activated membrane ATPase: Selective inhibition by ruthenium red. Biochim. Biophys. Acta 249, 606–610 (1971)
Wins, P., Schoffeniels, E.: Studies on red cell ghost ATPase systems: Properties of a (Mg2++Ca2+)-dependent ATPase. Biochim. Biophys. Acta 120, 341–350 (1966)
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Meltzer, H.L. The effect of ruthenium red and NEM on lithium efflux from human erythrocytes. Psychopharmacology 64, 25–30 (1979). https://doi.org/10.1007/BF00427340
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DOI: https://doi.org/10.1007/BF00427340