Abstract
A new yeast-like fungus isolated from jam on two occasions is described as Trichosporonoides nigrescens. It is a true xerophile, able to grow at water activities at least as low as 0.75. For this reason, its carbon assimilation pattern was examined using both dilute (standard) and concentrated carbon sources: six to nine compounds not assimilated at the standard concentrations of 0.5% (0.001 to 0.1 mol/kg) were assimilated at 1.5 to 2.0 mol/kg. Cell wall and septal ultrastructure, DNA base ratios, and the nuclear cycle were examined to determine the generic and higher taxonomic affinities of this microorganism. All properties were consistent with its assignment to Trichosporonoides Haskins et Spencer. However, while ultrastructure indicated an affinity with the Basidiomycetes, the mitotic nuclear cycle and the xerophilic nature of this fungus suggested an Ascomycete affinity. The GC content (57%) is consistent with assignment to either. No teleomorphic state was found.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
De Hoog, G. S. 1979a. Taxonomic review of Moniliella, Trichosporonoides and Hyalodendron. —Stud. Mycol., Baarn 19: 1–36.
De Hoog, G. S. 1979b. The taxonomic position of Moniliella, Trichosporonoides and Hyalodendron —an essay. — Stud. Mycol., Baarn 19: 81–90.
Dupont, P. F. and Hedrick, L. R. 1971. Deoxyribonucleic acid base composition and numerical taxonomy of yeasts in the genus Trichosporon. — J. Gen. Microbiol. 66: 349–359.
Haskins, R. H. 1975. Septal ultrastructure and hyphal branching in the pleomorphic imperfect fungus Trichosporonoides oedocephalis. — Can. J. Bot. 53: 1139–1148.
Kornerup, A. and Wanscher, J. H. 1978. Methuen Handbook of Colour, 3rd ed. — Eyre Methuen Ltd., London.
Kreger-van Rij, N. J. W. and Veenhuis, M. 1971. A comparative study of the cell wall structure of basidiomycetous and related yeasts. — J. Gen. Microbiol. 68: 87–95.
Martínez, A. T. 1979. Ultrastructure of Moniliella, Trichosporonoides and Hyalodendron. — Stud. Mycol., Baarn 19: 50–57.
Martínez, A. T., De Hoog, G. S., Smith, M. Th., Hogeweg, P. and Bruinsma, P. 1979. Physiological characteristics of Moniliella, Trichosporonoides and Hyalodendron. — Stud. Mycol., Baarn 19: 58–68.
McCully, E. K. and Robinow, C. F. 1972a. Mitosis in heterobasidiomycetous yeasts. I. Leucosporidium scottii (Candida scottii). — J. Cell Sci. 10: 857–881.
McCully, E. K. and Robinow, C. F. 1972b. Mitosis in heterobasidiomycetous yeasts. II. Rhodosporidium sp. (Rhodotorula glutinus) and Aessosporon salmonicolor (Sporobolomyces salmonicolor). — J. Cell Sci. 11: 1–31.
Norrish, R. S. 1966. An equation for the activity coefficients and equilibrium relative humidities of water in confectionery syrups. — J. Food Technol. 1: 25–39.
O'Connor, R. M., McArthur, C. R. and Clark-Walker, G. D. 1976. Respiratory-deficient mutants of Torulopsis glabrata, a yeast with circular mitochondrial deoxyribonucleic acid of 6 μm. — J. Bacteriol. 126: 959–968.
Pitt, J. I. 1973. An appraisal of identification methods for Penicillium species: novel taxonomic criteria based on temperature and water relations. — Mycologia 65: 1135–1157.
Pitt, J. I. 1975. Xerophilic fungi and the spoilage of foods of plant origin. p. 273–307. In R. B. Duckworth (ed.), Water relations of foods. — Academic Press, London.
Price, C. W., Fuson, G. F. and Phaff, H. J. 1978. Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Saccharomyces, Debaryomyces and Pichia. — Microbiol. Rev. 42: 161–193.
Radloff, R., Bauer, W. and Vinograd, J. 1967. A dye-buoyant-density method for the detection and isolation of closed circular DNA in HeLa cells. — Proc. Natl. Acad. Sci. U.S. 57: 1514–21.
Reynolds, E. S., 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. — J. Cell Biol. 17: 208–211.
Robinow, C. F. 1961. Mitosis in the yeast Lipomyces lipofer. — J. Biophys. Biochem. Cytol. 9: 879–892.
Schildkraut, C. L., Marmur, J. and Doty, P. 1962. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. — J. Mol. Biol. 4: 430–433.
Storck, R. and Alexopoulos, C. J. 1970. Deoxyribonucleic acid of fungi. — Bacteriol. Rev. 34: 126–154.
Storck, R., Alexopoulos, C. J. and Phaff, H. J. 1969. Nucleotide composition of deoxyribonucleic acid of some species of Cryptococcus, Rhodotorula and Sporobolomyces. — J. Bacteriol. 98: 1069–1072.
Tresner, H. D. and Hayes, J. A. 1971. Sodium chloride tolerance of terrestrial fungi. — Appl. Microbiol. 22: 210–213.
Van der Walt, J. P. 1970. Criteria and methods used in classification. p. 36–113. In J. Lodder, (ed.), The yeasts, a taxonomic study. — North Holland Publ. Co., Amsterdam.
Van der Walt, J. P. and Hopsu-Havu, V. K. 1976. A colour reaction for the differentiation of ascomycetous and hemibasidiomycetous yeasts. — Antonie van Leeuwenhoek 42: 157–163.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hocking, A.D., Pitt, J.I. Trichosporonoides nigrescens sp. nov., a new xerophilic yeast-like fungus. Antonie van Leeuwenhoek 47, 411–421 (1981). https://doi.org/10.1007/BF00426003
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00426003