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Identification of constitutive and herbicide inducible cytochromes P-450 in Streptomyces griseolus

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Abstract

The elevated soluble cytochrome P-450 content of Streptomyces griseolus cells found after growth in the presence of sulfonylurea herbicides has been shown to be the result of the appearance of one predominant cytochrome P-450 form. This cytochrome P-450 is the major soluble protein found to increase in amount following herbicide treatment, and functions as part of a sulfometuron methyl hydroxylase system. A second minor inducible cytochrome P-450 has been observed only in cells grown in the presence of chlorimuron ethyl, and a third cytochrome P-450 has been found to be present in all cells independent of the presence of sulfonylurea inducers. The three cytochrome P-450 isozymes are distinguishable primarily by their anion exchange properties; however, spectral properties, substrate inducibility, and enzymatic activity provide several further distinguishing features. The recognition of these inducible, xenobiotic metabolizing cytochromes P-450 in S. griseolus provides the only known description of monooxygenase proteins related to herbicide metabolism in bacteria.

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Abbreviations

Chlorsulfuron:

2-chloro-N-[4-methoxy-6-methyl-1,3,5-triazin-2-yl) aminocarbonyl]benzenesulfonamide

sulfometuron methyl:

N-[(4,6-dimethylpyrimidine-2-yl)aminocarbonyl]-2-methoxycarbonyl-benzenesulfonamide

chlorimuron ethyl:

N-[(4-chloro-6-methoxypyrimidine-2-yl)aminocarbonyl]2-ethoxycarbonylbenzene-sulfonamide

LDS:

lithium dodecyl sulfate

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Authors and Affiliations

  1. Central Research and Development Department, E. I. du Pont de Nemours and Company, Experimental Station, 19898, Wilmington, DE, USA

    Daniel P. O'Keefe & James A. Romesser

  2. Agricultural Products Department, E. I. du Pont de Nemours and Company, Experimental Station, 19898, Wilmington, DE, USA

    Kenneth J. Leto

Authors
  1. Daniel P. O'Keefe
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  2. James A. Romesser
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  3. Kenneth J. Leto
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O'Keefe, D.P., Romesser, J.A. & Leto, K.J. Identification of constitutive and herbicide inducible cytochromes P-450 in Streptomyces griseolus . Arch. Microbiol. 149, 406–412 (1988). https://doi.org/10.1007/BF00425579

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  • DOI: https://doi.org/10.1007/BF00425579

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