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Protein synthesizing systems from spores and vegetative cells of Bacillus cereus

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Abstract

A system of polyphenylalanine synthesis was optimized for a comparison of the polymerizing activities of ribosomes from spores and vegetative cells of Bacillus cereus T. Ribosomes of both types react similarly, showing a magnesium optimum of about 6 mM and spermidine optima of about 5 mM and 4 mM for vegetative and spore ribosomes, respectively. These lead to optimum mono- to multivalent cation rations of 9 and 10 respectively at 100 mM ammonium ion. A comparison of the response of these ribosomes to suboptimal concentrations of magnesium and spermidine show that they differ qualitatively from each other, suggesting that they possess different structure, macromolecular or ionic components.

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Abbreviations

DFP:

diisopropylfluorophosphate

HEPES:

N-2-hydroxyethylpiperazine-N′-ethanesulfonic acid

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Author notes

  1. R. A. Kieras

    Present address: Department of Cellular and Developmental Biology, Biological Sciences West, University of Arizona, 85721, Tucson, Arizona, USA

Authors and Affiliations

  1. Department of Cell and Molecular Biology, Division of Biological Sciences, University of Michigan, 48109, Ann Arbor, Michigan, USA

    H. A. Douthit, R. A. Kieras & R. A. Preston

Authors
  1. H. A. Douthit
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  2. R. A. Kieras
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  3. R. A. Preston
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Douthit, H.A., Kieras, R.A. & Preston, R.A. Protein synthesizing systems from spores and vegetative cells of Bacillus cereus . Arch. Microbiol. 121, 235–240 (1979). https://doi.org/10.1007/BF00425061

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  • DOI: https://doi.org/10.1007/BF00425061

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