Summary
MboI, HinfI and ThaI cleavage maps have been constructed for the region of the mitochondrial DNA from S. cerevisiae where transfer RNA genes are principally located. About 40 cleavage sites have been localized between the C and P genetic markers. The MboI map covers about 50% of the total mitochondrial genome. For constructing maps we have used a series of rho− deletion mutants whose mitochondrial DNAs have a typical single deletion structure as judged by previous genetic and physical analyses. The mutant DNAs carry known transfer RNA genes and genetic markers and, therefore, the comparison between genetic and restriction maps has allowed us to localize individual transfer RNA genes within defined physical segments.
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Abbreviations
- bp:
-
base pairs
- mtDNA:
-
mitochondrial DNA
- tRNA:
-
transfer RNA
- rRNA:
-
ribosomal RNA
- ThaI:
-
formerly TacI
References
Bolotin-Fukuhara M, Faye G, Fukuhara H (1977) Mol Gen Genet 152:295–305
Bos JL, Osinga KA, Van der Horst G, Borst P (1979) Nucl Acids Res 6:3255–3265
Casey J, Hsu JH, Rabinowitz M, Getz GS, Fukuhara H (1974) J Mol Biol 88:717–733
Colletti E, Frontali L, Palleschi C, Wésolowski M, Fukuhara H (1979) Mol Gen Genet 175:1–4
Fox TG (1979) Proc Nat Acad Sci USA 76:6534–6538
Fukuhara H, Bolotin-Fukuhara M, Hsu HJ, Rabinowitz M (1976) Mol Gen Genet 145:7–17
Hensgens LAM, Grivell LA, Borst P, Bos JL (1979) Proc Nat Acad Sci USA 76:1663–1667
Lewin A, Morimoto R, Rabinowitz M, Fukuhara H (1978) Mol Gen Genet 163:257–275
Li M, Tzagoloff A (1979) Cell 18:47–53
Macino G, Tzagoloff A (1979a) Mol Gen Genet 169:183–188
Macino G, Tzagoloff A (1979b) Proc Nat Acad Sci USA 76:131–135
Martin NC, Rabinowitz M, Fukuhara H (1977) Biochemistry 16:4672–4677
Martin NC, Miller D, Hartley J, Moynihan P, Donelson J (1980) Cell 19:339–343
Martin RP, Schneller JM, Stahl AJC, Dirheimer G (1977) Nucl Acids Res 4:3497–3510
Miller LD, Martin NC, Dinh Pham H, Donelson JE (1979) J Biol Chem 254:11735–11740
Miller DL, Sigurdson C, Martin NC, Donelson JE (1980) Nucl Acids Res
Morimoto R, Rabinowitz M (1979) Mol Gen Genet 170:11–23
Morimoto R, Lewin A, Merten S, Rabinowitz M (1976) Restriction endonuclease mapping and analysis of grande and mutant yeast mitochondrial DNA. In: Saccone C, Kroon AM (eds) The genetic function of mitochondrial DNA. Elsevier Publishers, North-Holland, Amsterdam, p 519
Morimoto R, Lewin A, Rabinowitz M (1977) Nucl Acids Res 4:2331–2351
Morimoto R, Merten S, Lewin S, Martin NC, Rabinowitz M (1978) Mol Gen Genet 163:241–255
Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes JC, Hutchison CA, Slocombe PM, Smith M (1977) Nature 265:687–695
Schweyen RJ, Weiss-Brummer B, Backhaus B, Kaudewitz F (1976) Localization of seven gene loci on a circular map of the mitochondrial genome of Saccharomyces cerevisiae. In. Saccone C, Kroon AM (eds). The genetic function of mitochondrial DNA. North-Holland Press, Amsterdam, p 251
Sutcliffe JG (1978) Nucl Acids Res 5:2721–2728
Tabak HF, Hecht NB, Menke HH, Hollenberg CP (1979) Curr Genet 1:33–43
Tzagoloff A, Foury F, Akai A (1976) Resolution of the mitochondrial genome. In: Saccone C, Kroon AM (eds). The genetic function of mitochondrial DNA. Elsevier/North-Holland Biomedical, Amsterdam, p 155
Van Ommen GJB, Groot GSP, Borst P (1977) Mol Gen Genet 154:255–262
Wésolowski M, Fukuhara H (1979) Mol Gen Genet 170:261–275
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Wésolowski, M., Monnerot, M. & Fukuhara, H. MboI, ThaI and HinfI endonuclease cleavage maps of the yeast mitochondrial DNA. Curr Genet 2, 121–129 (1980). https://doi.org/10.1007/BF00420624
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DOI: https://doi.org/10.1007/BF00420624