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Applications of modelling for bioprocess design and control in industrial production

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Abstract

The on-line measurement of the relevant parameters and the control conception for three production processes for fine chemicals by fermentation and biotransformation at the 15 m3 scale were developed. The models describe the bioprocesses which successfully result in fully automated manufacturing steps. Modelling also proved to be a valuable tool for a better insight into biochemical fundamentals of the processes. Moreover, proper use of data logging, modelling and process control was important for quality, since two processes were controlled on-line and quality relevant deviations were registered early. Finally, combining modelling with simulation, we could drastically reduce both development time and cost.

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Abbreviations

F l/h:

flux

V l:

volume

U 0 g/l:

nicotinonitrile concentration influx

U g/l:

actual nicotinonitrile concentration

q ug/gh:

specific educt (=nicotinonitrile) transformation rate

x g/l:

biocatalyst concentration

p 0 g/l:

nicotinamide concentration influx

p g/l:

actual nicotinamide concentration

q pg/gh:

specific product (=nicotinamide) formation rate

k :

parameter loss of activity

q u, maxg/gh:

max. specific educt transformation rate

K ug/l:

saturation constant for nicotinonitrile

K ig/l:

inhibition constant for nicotinonitrile

K iig/l:

inhibition constant for nicotinamide

MW Ag/mol:

molecular weight for nicotinonitrile

MW Bg/mol:

molecular weight for nicotinamide

NS :

Nicotinic acid

6-HNS:

6-Hydroxynicotinic acid

r NS, 6HNS g/lh:

6-HNS production rate

r 6HNS, X g/lh:

biomass production rate

r NS, 6HNS, max g/lh:

max. 6-HNS production rate

S NS g/l:

actual NS concentration

K S, NS g/l:

saturation constant for NS

K i, 6HNS g/l:

inhibition constant for 6-HNS

K o2 g/l:

saturation constant for oxygen

r 6HNS, X, max g/lh:

max. biomass production rate

S 6HNS g/l:

actual 6-HNS concentration

K ii, NS g/l:

inhibition constant for NS

RQ mol/mol:

respiration quotient

S xylg/l:

actual xylene concentration

K i, xylg/:

inhibition constant for xylene

K i, DMPYg/:

inhibition constant for 2,5-dimethylpyrazine

r Xg/lh:

biomass production rate

r X, maxg/lh:

max. biomass production rate

K s, xylg/l:

saturation constant for xylene

S DMPYg/l:

actual concentration of DMPY

K i, MPCAg/:

inhibition constant for MPCA

K O2g/:

saturation constant for oxygen

S MPCAg/l:

actual MPCA concentration

S O2g/l:

actual oxygen concentration

r MPCAg/lh:

MPCA production rate

r MPCA, maxg/lh:

max. MPCA production rate

k lgl:

inhibition constant for the intermediates

k s, DMPYgl:

saturation constant for DMPY

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Authors and Affiliations

  1. Bioprocess Development, LONZA AG, CH-3930, Visp, Switzerland

    M. Rohner & H. -P. Meyer

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  1. M. Rohner
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  2. H. -P. Meyer
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Rohner, M., Meyer, H.P. Applications of modelling for bioprocess design and control in industrial production. Bioprocess Engineering 13, 69–78 (1995). https://doi.org/10.1007/BF00420432

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