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Characterization of a new Bacillus megaterium bacteriophage, MJ-1, from tropical soil

  • Genetics and Molecular Biology
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Abstract

A new Bacillus megaterium bacteriophage is characterized. It is a tailed phage with regular polyhedral head belonging to Bradley's group B. Head and tail dimensions are 56.4 and 300 nm, respectively. Lysis was restricted to strains of B. megaterium. No antigenic relationship with pumilus phage FP-1 or subtilis phage FS-1 was observed. The phage is sensitive to 60°C and moderately sensitive to chloroform. The nucleic acid is double-stranded linear DNA with a G-C mole % of 38.8 and a mol wt of (53±3)×106.

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References

  • Ackermann H. W. 1974. La classification des bacteriophages de Bacillus et Clostridium. — Pathol. Biol. 22: 909–917.

    Google Scholar 

  • Ackermann H. W., Audurier A., Aerthiaume L., Jones L. A., Mayo J. A. and Vidaver A. K. 1978. Guidelines for bacteriophage characterization. — Adv. Virus Res. 23: 1–23.

    Google Scholar 

  • Adams M. H. 1959. Bacteriophages. — Interscience Publishers, New York.

    Google Scholar 

  • Aradley D. E. 1967. Ultrastructure of bacteriophages and bacteriocins. — Bacteriol. Rev. 31: 230–314.

    Google Scholar 

  • Carvalho P. M. and Vary J. C. 1977. Isolation and characterization of a Bacillus megaterium QM B1551 bacteriophage. — J. Gen. Virol. 36: 547–550.

    Google Scholar 

  • Cassity T. R. and Kolodziej B. J. 1979. Partial characterization of a new Bacillus megaterium bacteriophage and inhibition of bacteriophage adsorption by B. megaterium capsule. — FEMS Microbiol. Lett. 5: 61–63.

    Google Scholar 

  • Cooney P. H., Jacob R. J. and Slepecky R. A. 1975. Characteristics of a Bacillus megaterium bacteriophage. — J. Gen. Virol. 26: 131–134.

    Google Scholar 

  • Ehrlich H. L. and Pfau C. J. 1957. Studies of a plaque variation of Bacillus megaterium phage. — Can. J. Microbiol. 3: 1011–1020.

    Google Scholar 

  • Friedman M. and Cowles P. B. 1953. The bacteriophages of Bacillus megaterium. I. Serological, physical and biological properties. — J. Bacteriol. 66: 379–385.

    Google Scholar 

  • Gordon R. E., Haynes W. C. and Pang C. H.-N. 1973. The Genus Bacillus. Agriculture Handbook 427. — US Department of Agriculture, Washington D.C.

    Google Scholar 

  • Hendry G. S. and Fitz-James P. C. 1974. Characteristics of øT, the temperate bacteriophage carried by Bacillus megaterium 899a. — J. Virol. 13: 494–499.

    Google Scholar 

  • Hirschmann S. Z. and Felsenfeld G. 1966. Determination of DNA composition and concentration by spectral analysis. — J. Mol. Biol. 16: 347–358.

    Google Scholar 

  • Kleinschmidt A. K. 1968. Monolayer techniques in electron microscopy of nucleic acid molecules. p. 361–377. In S. P.Colowick and N. O.Kaplan (eds.), Methods in enzymology. Vol. XIIB. — Academic Press, New York and London.

    Google Scholar 

  • Lang D. 1970. Molecular weights of coliphages and coliphage DNA. — J. Mol. Biol. 54: 557–565.

    Google Scholar 

  • Marmur J. 1961. A procedure for the isolation of deoxyribonucleic acid from microorganisms. — J. Mol. Biol. 3: 208–218.

    Google Scholar 

  • Marmur J. and Doty P. 1962. Determination of base composition of deoxyribonucleic acid from its thermal denaturation temperature. — J. Mol. Biol. 5: 109–118.

    Google Scholar 

  • Schildkraut C. L., Marmur J. and Doty P. 1962. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. — J. Mol. Biol. 4: 430–443.

    Google Scholar 

  • Scott I. R., Stewart G. S. A. G., Koncewicz M. A., Ellar D. J. and Craftslighty A. 1978. Sequence of biochemical events during germination of Bacillus megaterium spores. p. 95–103. In G.Chambliss and J. C.Vary (eds), Spores. Vol. VII. — American Society for Microbiology, Washington D.C.

    Google Scholar 

  • Steensma H. Y., Robertson L. A. and van Elsas J. D. 1978. The occurrence and taxonomic value of PBSX-like defective phages in the genus Bacillus. — Antonie van Leeuwenhoek 44: 353–366.

    Google Scholar 

  • Steensma H. Y. and Sondermeijer P. J. A. 1977. A counting method for determining the burst size of defective phages from Bacillus subtilis. — Antonie van Leeuwenhoek 43: 305–316.

    Google Scholar 

  • Van Elsas J. D. and Penido E. G. C. 1981a. Isolation and characterization of Bacillus pumilus phages from Brazilian soil. — Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. II Abt. 136: 581–589.

    Google Scholar 

  • Van Elsas J. D. and Penido E. G. C. 1981b. Characterization of phages of Bacillus subtilis isolated from Brazilian soils. — Rev. Microbiol. 12(2): 49–54.

    Google Scholar 

  • Van Elsas J. D., Seldin L. and Penido E. G. C. 1978. Ecology and taxonomy of Bacillus in Brazilian soils. — Anais de Microbiologia (Rio de Janeiro) 23: 59–78.

    Google Scholar 

  • Vary P. S. 1979. Transduction in Bacillus megaterium. — Bioch. Bioph. Res. Commun. 88: 1119–1124.

    Google Scholar 

  • Yamamoto K. R., Alberts B. M., Aenziger R., Lawhorne L. and Treiber G. 1970. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to largescale virus purification. — Virology 40: 734–744.

    Google Scholar 

  • Yehle C. O. and Doi R. H. 1967. Differential expression of bacteriophage genomes in vegetative and sporulating cells of Bacillus subtilis. — J. Virol. 1: 935–947.

    Google Scholar 

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Authors and Affiliations

  1. Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I, Ilha do Fundão, 21.941, Rio de Janeiro, RJ, Brasil

    J. D. van Elsas & E. G. C. Penido

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  1. J. D. van Elsas
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  2. E. G. C. Penido
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van Elsas, J.D., Penido, E.G.C. Characterization of a new Bacillus megaterium bacteriophage, MJ-1, from tropical soil. Antonie van Leeuwenhoek 48, 365–371 (1982). https://doi.org/10.1007/BF00418289

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  • DOI: https://doi.org/10.1007/BF00418289

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