Skip to main content
Log in

Measurement variability analysis for fermentation process assays

  • Originals
  • Published:
Bioprocess Engineering Aims and scope Submit manuscript

Abstract

The paper reports the variability of replicate measurements for soluble protein and some enzyme activities in batch and continuous culture of S. cerevisiae. The measurement variability in these assays depended on the measured concentration and could be represented as a standard deviation proportional to the measured value.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Schaaff, I.; Heinisch, J.; Zimmermann, F.K.: 1989, Overproduction of glycolitic enzymes in yeast, Yeast 5, 285–290

    Google Scholar 

  2. Postma, E.; Verduyn, C; Scheffers, A.; Van Dijken, J.P.: 1989, Enzymatic analysis of the Crabtree effect in glucose-limited chemostat cultures, Appl. Env. Micro., 55, 468–477

    Google Scholar 

  3. Verduyn, C.; Postma, E.; Scheffers, A.; Van Dijken, J.P.: 1990, Physiology of S. cerevisiae in aerobic glucose-limited chemostat cultures, J. Gen. Microbiol., 136, 395–403

    Google Scholar 

  4. Maitra, P.K.; Lobo, Z.: 1971, A kinetic study of glycolitic enzyme synthesis in yeast, J. Biol. Chem., 246, 475–488

    Google Scholar 

  5. Bradford, M.M.: 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding, Analytical Biochem., 72, 248–254

    Google Scholar 

  6. Clarkson, A.I.; Bulmer, M.; Siddiqi, S.F.; Titchener-Hooker, N.J.: 1994, Pilot scale verification of bioprocess models, Comput. Chem. Engng., 18, 651–655

    Google Scholar 

  7. Stone, K.; Roche, F.W.; Thornhill, N.F.: 1992, Dry weight measurement of microbial biomass and measurement variability analysis, Biotechnol. Techniques., 6(3), 207–212

    Google Scholar 

  8. Fiechter, A.; Fuhrmann, G.F.; Kapelli, O.: 1981 Regulation of glucose metabolism in growing yeast cells, Adv. Micro. Physiol., 22, 123–183

    Google Scholar 

  9. Bergmeyer, H.U.: 1983, Methods of enzymic analysis Vol. 1, (3rd Edition), Verlag Chemie, Weheim

    Google Scholar 

  10. Bulmer, M.: 1992, PhD Thesis, University of London

  11. Darley-Usmar, V.M.; Ricjwood, D.; Wilson, M.T.: 1987, Mitochondria: A Practical Approach, IRL Press, Oxford

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

The support of the ESPRC and BBSRC through the Interdisciplinary Research Centre for Process Systems Engineering, Imperial College of Science, Technology and Medicine, and through the Advanced Centre for Biochemical Engineering, University College London is gratefully acknowledged.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dehghani, M., Bulmer, M., Gregory, M.E. et al. Measurement variability analysis for fermentation process assays. Bioprocess Engineering 13, 239–243 (1995). https://doi.org/10.1007/BF00417634

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00417634

Keywords

Navigation