Abstract
Addition of the arginine analogue, canavanine, to cultures of nitrogen-fixing Anabaena cylindrica at the onset of akinete formation, resulted in the development of akinetes randomly distributed within the filament, in addition to those adjacent to heterocysts. The total frequency of akinetes increased up to five-fold. A feature of akinetes is their increased content of cyanophycin granules (an arginine-aspartic acid polymer) and addition of canavanine to cultures at an earlier stage resulted in entire filaments becoming agranular and containing agranular akinetes. The effects on akinete pattern appeared to be specific for canavanine since other amino acid analogues, although increasing the frequency of akinetes (approximately two-fold), had no effect on their position relative to heterocysts. In ammonia-grown, stationary phase cultures of A. cylindrica, akinetes were observed adjacent to proheterocysts and in positions more than 20 cells from any heterocyst. These observations indicate that nitrogen fixation and heterocysts are not essential for akinete formation in A. cylindrica, although the availability of a source of fixed nitrogen does appear to be a requirement.
These results suggest that during exponential growth some aspect of the physiology of vegetative cells suppresses their development into akinetes and that the role of the heterocyst may not be one of direct stimulation of adjacent vegetative cells to form akinetes, but the removal or negation of the inhibition within them. A model for akinete formation and the involvement of canavanine is given.
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References
Allen, M. B., Arnon, D. I.: Studies on nitrogen-fixing blue-green algae. I. Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant. Physiol. 30, 366–372 (1955)
Baker, J. R.: The histochemical recognition of certain guanidine derivatives. Q. J. Microsc. Sci. 88, 115–121 (1947)
Bone, D. H.: The influence of canavanine, oxygen and urea on the steady-state levels of nitrogenase in Anabaena cylindrica. Arch. Mikrobiol. 86, 13–24 (1972)
Bradley, S., Carr, N. G.: Heterocyst and nitrogenase development in Anabaena cylindrica. J. Gen. Microbiol. 96, 175–184 (1976)
Burger, J.: A study of two populations of Anabaena planctonica Brunnth. (cyanophyta) from Minnesota. Phycologia 13, 125–129 (1974)
Eberly, W. R.: Notes on some new and rare Myxophyceae in laboratory culture. Trans. Am. Microsc. Soc. 85, 130–138 (1966)
Fisher, R. W., Wolk, C. P.: Substance stimulating the differentiation of spores of the blue-green alga Cylindrospermum licheniforme. Nature 259, 394–395 (1976)
Hill, H.: A new form form of Rhaphidiopsis mediterranea Skuja found in Minnesota lakes. Phycologia 9, 73–77 (1970)
Miller, M. M., Lang, N. J.: The fine structure of akinete formation and germination in Cylindrospermum. Arch. Mikrobiol. 60, 303–313 (1968)
Mitchison, G. J., Wilcox, M.: Alteration in heterocyst pattern of Anabaena produced by 7-azatryptophan. Nature New Biol. 246, 229–233 (1973)
Nichols, J. M., Carr, N. G.: Akinetes of cyanobacteria. In: Spores VII (G. Chambliss, J. C. Vary, eds.), pp. 335–343. Washington: Am. Soc. Microbiol. 1978
Simon, R. D.: Cyanophycin granules from the blue-green alga Anabaena cylindrica: A reserve material consisting of copolymers of aspartic acid and arginine. Proc. Natl. Acad. Sci. USA 68, 265–267 (1971)
Simon, R. D.: Sporulation in the filamentous cyanobacterium Anabaena cylindrica. The course of spore formation. Arch. Microbiol. 111, 283–288 (1977)
Singh, H. N., Srivastava, B. S.: Studies on morphogenesis in a blue-green alga. I. Effect of inorganic nitrogen sources on developmental morphology of Anabaena doliolum. Can. J. Microbiol. 14, 1341–1346 (1968)
Sutherland, J. M., Herdman, M., Stewart, W. D. P.: Akinetes of the cyanobacterium Nostoc PCC 7524: Macromolecular composition, structure and control of differentiation. J. Gen. Microbiol. 115, 273–287 (1979)
Tyagi, V. V. S.: Some observations on the pattern of sporulation in a blue-green alga, Anabaena doliolum. Ann. Bot. 38, 1107–1111 (1974)
Tyagi, V. V. S.: The pattern of sporulation in Anabaena circinalis and comments on the role of heterocysts in sporulation in blue-green algae. Z. Allg. Mikrobiol. 18, 281–286 (1978)
Wildman, R. B., Loescher, J. H., Winger, C. L.: Development and germination of akinetes of Aphanizomenon flos-aquae. J. Phycol. 11, 96–104 (1975)
Wolk, C. P.: Control of sporulation in a blue-green alga. Dev. Biol. 12, 15–35 (1965)
Wolk, C. P.: Evidence of a role of heterocysts in the sporulation of a blue-green alga. Am. J. Bot. 53, 260–262 (1966)
Wolk, C. P., Austin, S. M., Bortins, J., Galonsky, A.: Autoradiographic localization of 13N after fixation of 13N-labeled nitrogen gas by a heterocyst-forming blue-green alga. J. Cell Biol. 61, 440–453 (1974)
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Nichols, J.M., Adams, D.G. & Carr, N.G. Effect of canavanine and other amino acid analogues on akinete formation in the cyanobacterium Anabaena cylindrica . Arch. Microbiol. 127, 67–75 (1980). https://doi.org/10.1007/BF00414357
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DOI: https://doi.org/10.1007/BF00414357