Summary
Direct selection for TRP+ transformants of a trp-2 mutant of Coprinus cinereus showed that the gene mutation could be complemented by the heterologous gene from two other basidiomycete species, Schizophyllum commune (trp1) and Phanerochaete chrysosporium (trpC) but not by an ascomycete gene from Aspergillus nidulans (trpC). Cotransformation was used to confirm that the Aspergillus nidulans gene could be integrated but not expressed. Cotransformations were also used to show that the Aspergillus nidulans isocitrate lyase gene (acuD) and a construct containing the bacterial HygB gene fused to a hemibasidiomycete gene promotor from Ustilago maydis were also unexpressed in Coprinus cinereus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Apirion D (1962) Genet Res 6:317–329
Ballance DJ, Turner G (1986) Mol Gen Genet 202:271–276
Beri RK, Turner G (1987) Curr Gen 11:639–641
Binninger DM, Skrzynia C, Pukkila PJ, Casselton LA (1987) EMBO J 6:835–840
Brown A, Sims PFG, Raeder U, Broda P (1988) Gene 73:77–85
Casselton LA, Casselton PJ (1974) Mol Gen Genet 132:255–264
Froeliger EH, Munoz-Rivas AM, Specht CA, Ullrich RC, Novotny CP (1987) Curr Genet 12:547–554
Gurr SJ, Unkles SE, Kinghorn JR (1987) In: Kinghorn JR (ed) Gene structure of eukaryotic microbes. IRL Press Oxford, pp 93–139
Hamer JE, Timberlake WE (1987) Mol Cell Biol 7:2352–2359
Hütter R, Niederberger P, DeMoss JA (1986) Annu Rev Microbiol 40:55–77
Hynes MJ (1978) J Bacteriol 131:770–775
Hynes MJ (1989) Exp Mycol 13:196–198
Keesey JR, DeMoss JA (1982) J Bacteriol 152:954–958
Kelly JM, Hynes MJ (1985) EMBO J 4:475–479
Mellon FM, Casselton LA (1988) Cur Genet 14:451–456
Mellon FM, Little PFR, Casselton LA (1987) Mol Gen Genet 210:352–357
Munoz-Rivas AM, Specht CA, Ullrich RC, Novotny CP (1986) Curr Genet 10:909–913
Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, Van den Hondel CAMJJ (1987) Gene 56:117–124
Rambosek J, Leach J (1987) C R C Critical Rev Biotech 6:357–393
Rao PS, Niederpruem DJ (1969) J Bacteriol 100:1222–1228
Revuelta JL, Jayaram M (1987) Mol Cell Biol 7:2664–2670
Rodriquez RJ, Yoder OC (1987) Gene 54:73–81
Sanchez F, Tourino A, Traseira S, Perez-Aranda A, Rubio V, Penalva MA (1986) Mol Gen Genet 205:248–252
Schechtman MG, Yanofsky C (1983) J Mol Appl Genet 2:83–89
Sealy Lewis HM, Casselton LA (1978) Mol Gene Genet 151:189–195
Sims P, James C, Broda P (1988) Gene 74:411–422
Tilburn J, Scazzochio C, Taylor GG, Zabicky-Zissman JH, Lockington RA, Davies RW (1983) Gene 26:205–221
Tilby MJ (1976) J Gen Microbiol 93:126–132
Turgeon BG, Garber RC, Yoder OC (1985) Mol Gen Genet 201:450–453
Turgeon BG, Macrae WD, Garber RC, Fink GR, Yoder OC (1986) Gene 42:79–88
Vieira J, Messing J (1982) Gene 19:259–268
Wang L, Holden WD, Leong SA (1988) Proc Natl Acad Sci USA 85:856–869
Yelton MM, Hamer JE, Timberlake WE (1984) Proc Natl Acad Sci USA 81:1470–1474
Zolan ME, Pukkila PJ (1986) Mol Cell Biol 6:195–200
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Casselton, L.A., de la Fuente Herce, A. Heterologous gene expression in the basidiomycete fungus Coprinus cinereus . Curr Genet 16, 35–40 (1989). https://doi.org/10.1007/BF00411081
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00411081