Abstract
Growth of various bacteria, especially aerobic hydrogen-oxidizing bacteria, in the presence of 2 to 100% (v/v) oxygen in the gas atmosphere was evaluated. The bacterial strains included Alcaligenes eutrophus, A. paradoxus, Aquaspirillum autotrophicum, Arthrobacter spec. strain 11X, Escherichia coli, Arthrobacter globiformis, Nocardia opaca, N. autotrophica, Paracoccus denitrificans, Pseudomonas facilis, P. putida, and Xanthobacter autotrophicus. Under heterotrophic conditions with fructose or gluconate as substrates neither colony formation on solid medium nor the growth rates in liquid media were drastically impaired by up to 100% oxygen. In contrast, autotrophic growth — with hydrogen, carbon dioxide and up to 80% oxygen in the gas atmosphere — was strongly depressed by high oxygen concentrations. However, only the growth rate, not the viability of the cells, was decreased. Growth retardation was accompanied by a decrease of hydrogenase activity.
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Aragno, M. and Schlegel, H. G. 1978. Physiological characterization of the hydrogen bacterium Aquaspirillum autotrophicum. — Arch. Microbiol. 116: 221–229.
Berndt, H., Ostwal, K.-P., Lalucat, J., Schumann, C., Mayer, F. and Schlegel, H. G. 1976. Identification and physiological characterization of the nitrogen fixing bacterium Corynebacterium autotrophicum GZ29. — Arch. Microbiol. 108: 1–26.
Biggins, D. R. and Postgate, J. R. 1969. Nitrogen fixation by cultures and cell-free extracts of Mycobacterium flavum 301. — J. Gen. Microbiol. 56: 181–193.
Dalton, H. 1980. Chemoautotrophic nitrogen fixation. p. 177–195. In W. D. Stewart and J. R. Gallon (eds), Nitrogen fixation. Annual Proceedings of the Phytochemical Society of Europe. Vol. 18. — Academic Press, London.
Eberhardt, U. 1969. On chemolithotrophy and hydrogenase of a gram-positive Knallgas bacterium. — Arch. Mikrobiol. 66: 91–104.
Fridovich, I. 1974. Superoxide and evolution. Horizons. — Biochem. Biophys. 1: 1–37.
Gottlieb, S. F. 1971. Effect of hyperbaric oxygen on microoganisms. — Annu. Rev. Microbiol. 25: 111–152.
Harrison, D. E. F. 1973. Growth, oxygen, and respiration. — Crit. Rev. Microbiol. 2: 185–228.
Hassan, H. M. and Fridovich, I. 1979. Superoxide dismutase and its role for survival in the presence of oxygen. p. 179–193. In S. Bernhard (ed.), Life Sciences Research Report 13. M. Shilo (ed.), Strategies of microbial life in extreme environments. — Verlag Chemie, Weinheim.
Ibrahim, M. and Schlegel, H. G. 1980. Oxygen supply to bacterial suspensions of high cell densities by hydrogen peroxide. — Biotechnol. Bioeng. 22: 1877–1894.
Jørgensen, B. B., Revsbech, N. P., Blackburn, T. H. and Cohen, Y. 1979. Diurnal cycle of oxygen and sulfide microgradients and microbial photosynthesis in a cyanobacterial mat sediment. — Appl. Environ. Microbiol. 38: 46–58.
King, W. R. and Andersen, K. 1980. Efficiency of CO2 fixation in a glycollate oxidoreductase mutant of Alcaligenes eutrophus which exports fixed carbon as glycollate. — Arch. Microbiol. 128: 84–90.
Krieg, N. R. 1976. Biology of the chemoheterotrophic spirilla. — Bacteriol. Rev. 40: 55–115.
Lorimer, G. H. and Andrews, T. J. 1981. The C2 photo- and chemo-respiratory carbon oxidation cycle. p. 329–374. In M. D. Hatch and N. K. Boardman (eds), The biochemistry of plants. Vol. 8. — Academic Press, New York.
Malik, K. A., Jung, C., Claus, D. and Schlegel, H. G. 1981. Nitrogen fixation by the hydrogen-oxidizing bacterium Alcaligenes latus. — Arch. Microbiol. 129: 254–256.
Morris, J. G. 1975. The physiology of obligate anaerobiosis. — Adv. Microb. Physiol. 12: 168–246.
Morris, J. G. 1976. Oxygen and the obligate anaerobe. — J. Appl. Bacteriol. 40: 229–244.
Morris, J. G. 1979. Nature of oxygen toxicity in anaerobic microorganisms. p. 149–162. In S. Bernhard (ed.), Life Sciences Research Report 13. M. Shilo (ed.), Strategies of microbial life in extreme environments. — Verlag Chemie, Weinheim.
Mulder, E. G. and Brotonegoro, S. 1974. Free-living heterotrophic nitrogen-fixing bacteria. p. 37–85. In A. Quispel (ed.), The biology of nitrogen fixation. — North-Holland, Amsterdam.
Okon, Y., Houchins, J. P., Albrecht, S. L. and Burris, R. H. 1977. Growth of Spirillum lipoferum at constant partial pressures of oxygen, and the properties of its nitrogenase in cell-free extracts. — J. Gen. Microbiol. 98: 87–93.
Schlegel, H. G. 1977. Aeration without air: oxygen supply by hydrogen peroxide. — Biotechnol. Bioeng. 19: 413–424.
Schlegel, H. G., Ibrahim, M. E. L., Wilde, E., Schneider, K., Schlesier, M., Friedrich, B. and Malik, K. A. 1981. Detrimental and beneficial effects of oxygen exerted on hydrogen-oxidizing bacteria. p. 107–129. In J. M. Lyons, R. C. Valentine, D. A. Phillips, D. W. Rains and R. C. Huffaker (eds), Genetic engineering of symbiotic nitrogen fixation. — Plenum Press, New York.
Schlegel, H. G., Kaltwasser, H. und Gottschalk, G. 1961. Ein Submersverfahren zur Kultur wasserstoffoxydierender Bakterien: Wachstumsphysiologische Untersuchungen. — Arch. Mikrobiol. 38: 209–222.
Schlegel, H. G. and Schneider, K. 1978. Introductory report: distribution and physiological role of hydrogenases in microorganisms. p. 15–44. In H. G. Schlegel and K. Schneider (eds), Hydrogenases: their catalytic activity, structure and function. — Verlag Erich Goltze KG., Göttingen.
Schlesier, M. and Friedrich, B. 1981. In vivo inactivation of soluble hydrogenase of Alcaligenes eutrophus. — Arch. Microbiol. 129: 150–153.
Schneider, K. and Schlegel, H. G. 1981. Production of superoxide radicals by soluble hydrogenase from Alcaligenes eutrophus H16. — Biochem. J. 193: 99–107.
Wimpenny, J. W. T. 1969. Oxygen and carbon dioxide as regulators of microbial growth and metabolism. — Symp. Soc. Gen. Microbiol. 19: 161–197.
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The work was supported by the Deutsche Forschungsgemeinschaft.
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Wilde, E., Schlegel, H.G. Oxygen tolerance of strictly aerobic hydrogen-oxidizing bacteria. Antonie van Leeuwenhoek 48, 131–143 (1982). https://doi.org/10.1007/BF00405198
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DOI: https://doi.org/10.1007/BF00405198