Summmary
Cell lysates from transformed hamster dermal fibroblasts (HDF cells) induced colony formation of normal HDF cells in soft agar medium and also stimulated proliferation of normal HDF cells in medium supplemented with plasma-derived serum (PDS). The colony forming activites of cell lysates correlated well with their growth stimulating activities in PDS-supplemented medium. The growth factor(s) were shown to be sensitive to trypsin and dithiothreitol (DTT), but heat stable. However, these transformed HDF cell lines did not all produce tumor nodules when injected into hamsters. This was not due to immunological rejection because similar results were obtained in hamsters treated with anti-hamster thymocyte serum (ATS). Thus it was concluded that production of these growth factor(s) by transformed HDF cell lines is not itself sufficient for acquisition of complete malignancy in vivo.
References
Katoh Y, Takayama S (1981) The growth responses of hamster chondrocytes, dermal fibroblasts and embryo cells to whole blood serum and plasma-derived serum. Experientia 37:386–387
Katoh Y, Takayama S (1982) Conversion of platelet-derived growth factor-dependent cells to growth factor-independent cells during chemical carcinogenesis in vitro. Carcinogenesis 3:867–873
Katoh Y, Takayama S (1984) Characterization of the growth responses of hamster fibroblasts and chondrogenic cells to fibroblast growth factor. Exp Cell Res 150:131–140
Koler N, Lipton A (1974) Platelets as a source of fibroblast growth promoting activity. Exp Cell Res 87:297–301
Moses HL, Branum EL, Proper JA, Robinson RA (1981) Transforming growth factor production by chemically transformed cells. Cancer Res 41:2842–2848
Roberts AB, Lamb LC, Newton DL, Sporn MB, Delarco JE, Todaro GJ (1980) Transforming growth factor: Isolation of polypeptides from virally and chemically transformed cells by acid/ ethanol extraction. Proc Natl Acad Sci USA 77:3494–3498
Ross R, Vogel A (1978) The platelet-derived growth factor. Cell 14:203–210
Ross R, Glosmet J, Kariya B, Harkar J (1974) A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc Natl Acad Sci USA 71:1207–1210
Ross R, Nist C, Kariya B, Rivest MJ, Raines E, Callis J (1978) Physiological quiescence in plasma-derived serum: Influence of platelet-derived growth factor on cell growth in culture. J Cell Physiol 97:497–508
Sakakibara K, Oota K (1975) Metastasis formation of Yoshida Sarcoma heteroplanted in adult golden hamsters treated with antihamster thymocyte serum. Cancer Res 35:548–553
Scher CD, Schepard RC, Antoniades HN, Stiles CD (1979) Platelet-derived growth factor and regulation of the mammalian fibroblast cell cycle. Biochim Biophys Acta 560:217–241
Stanbridge EJ, Der CJ, Doersen C-J, Nishimi RY, Peehl DM, Weissmann BE (1982) Human cell hybrids: Analysis of transformation and tumorigenicity. Science 215:252–259
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Katoh, Y. Characterization of growth factor(s) produced by chemically transformed hamster dermal fibroblasts. J Cancer Res Clin Oncol 110, 177–180 (1985). https://doi.org/10.1007/BF00402736
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DOI: https://doi.org/10.1007/BF00402736