Abstract
The incorporation of [1-3H] geranylgeranyl diphosphate (GGPP), [1-3H] geranylgeranyl monophosphate (GGMP) and [U-14C] phytyl diphosphate (PhPP) into chlorophylls a and b in growing tobacco cell cultures was investigated. The substrates were effectively incorporated into chlorophylls a and b, 3.2% of the total activity of applied GGPP or GGMP and 12.4% of the total activity of applied PhPP being found in chlorophylls a and b after 24 h incubation. The radioactivity was found in phytyl chlorophyllide through-out which means effective hydrogenation of the alcohol moiety in the case of GGPP and GGMP. With increasing substrate concentration, the specific radioactivity of chlorophyll increased up to a saturation level which was reached either at 20–40 μM PhPP or at 60 μM GGPP and GGMP. The specific radioactivity of the chlorophyll formed during the 24-h incubation period was the same as that of the applied substrate at saturating substrate concentration. The specific radioactivity of chlorophyll a was higher than that of chlorophyll b only in the case of PhPP.
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Abbreviations
- Chlide:
-
chlorophyllide a
- ChlPh :
-
phytyl chloro-phyllide
- ChlGG :
-
geranylgeranyl chlorophyllide a
- GGPP:
-
geranylgeranyl diphosphate
- GGMP:
-
geranylgeranyl monophosphate
- HPLC:
-
high-performance liquid chromatography
- PhPP:
-
phytyl diphosphate
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Dedicated to Professor Dr. Hubert Ziegler on the occasion of his 60th birthday
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Benz, J., Lempert, U. & Rüdiger, W. Incorporation of phytol precursors into chlorophylls of tobacco cell cultures. Planta 162, 215–219 (1984). https://doi.org/10.1007/BF00397442
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DOI: https://doi.org/10.1007/BF00397442