Abstract
Sulphur dioxide inhibits noncyclic photophosphorylation in isolated envelope-free chloroplasts. This inhibition was shown to be reversible and competitive with phosphate, with an inhibitor constant of Ki=0.8mM. The same inhibition characteristics were observed when phosphoglycerate (PGA)- or ribulose-1,5-bisphosphate (RuBP)-dependent oxygen evolution was examined in a reconstituted chloroplast system in the presence of SO 2-3 . Using an ATP-regenerating system (phosphocreatine-creatine kinase), it was demonstrated that the inhibition of PGA-dependent oxygen evolution is solely the result of inhibited photophosphorylation. It is concluded that at low SO2 and SO 2-3 concentrations the inhibition of photophosphorylation is responsible for the inhibition of photosynthetic oxygen evolution.
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Abbreviations
- Chl:
-
chlorophyll
- PGA:
-
D-3-phosphoglyceric acid trisodium salt
- Pi:
-
inorganic phosphate
- RuBP:
-
D-ribulose-1,5-bisphosphoric acid tetrasodium salt
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Cerović, Z.G., Kalezić, R. & Plesničar, M. The role of photophosphorylation in SO2 and SO 2-3 inhibition of photosynthesis in isolated chloroplasts. Planta 156, 249–254 (1982). https://doi.org/10.1007/BF00393732
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DOI: https://doi.org/10.1007/BF00393732