Abstract
We have compared the ability of shadegrown clones of Solamum dulcamara L. from shade and sun habitats to acclimate to bright light, as a function of nitrogen nutrition before and after transfer to bright light. Leaves of S. dulcamara grown in the shade with 0.6 mM NO -3 have similar photosynthetic properties as leaves of plants grown with 12.0 mM NO -3 . When transferred to bright light for 1–2 d the leaves of these plants show substantial photoinhibition which is characterized by about 50% decrease in apparent quantum yield and a reduction in the rate of photosynthesis in air at light saturation. Photoinhibition of leaf photosynthesis is associated with reduction in the variable component of low-temperature fluorescence emission, and with loss of in-vitro electron transport, especially of photosystem II-dependent processes.
We find no evidence for ecotypic differentiation in the potential for photosynthetic acclimation among shade and sun clones of S. dulcamara, or of differentiation with respect to nitrogen requirements for acclimation. Recovery from photoinhibition and subsequent acclimation of photosynthesis to bright light only occurs in leaves of plants provided with 12.0 mM NO -3 . In these, apparent quantum yield is fully restored after 14 d, and photosynthetic acclimation is shown by an increase in light-saturated photosynthesis in air, of light-and CO2-saturated photosynthesis, and of the initial slope of the CO2-response curve. The latter changes are highly correlated with changes in ribulose-bisphosphate-carboxylase activity in vitro. Plants supplied with 0.6 mM NO -3 show incomplete recovery of apparent quantum yield after 14 d, but CO2-dependent leaf photosynthetic parameters return to control levels.
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Abbreviations
- Fo :
-
initial level of fluorescence at 77 K
- Fm :
-
maximum level of fluorescence at 77 K
- Fv :
-
variable components of fluorescence at 77 K (Fv=Fm-Fo)
- PSI, PSII:
-
photosystem I and II, respectively
- RuBP:
-
ribulose-1,5-bisphosphate
- RuBPCase:
-
ribulose-1,5-bisphosphate carboxylase-oxygenase (EC 4.1.1.39)
References
Björkman, O. (1981) Responses to different quantum flux densities. In: Encyclopedia of plant physiology, N.S. vol. 12B: Physiological plant ecology I, pp. 47–107, Lange, O.L., Nobel, P.S., Osmond, C.B., Zeigler, H., eds. Springer-Verlag, Berlin Heidelberg New York
Björkman, O., Boardman, N.K., Anderson, J.M., Thorne, S.W., Goodchild, D.J., Pyliotis, N.A. (1972) Effects of light intensity during growth of Atriplex patula on the capacity of photosynthetic reactions, chloroplast components and structure. Carnegie Inst. Washington Yearb. 71, 115–135
Clough, J.M., Alberte, R.S., Teeri, J.A. (1980) Photosynthetic adaptation of Solanum dulcamara L. to sun and shade environments. III. Characterization of genotypes with differing photosynthetic performance. Oecologia 44, 221–225
Clough, J.M., Teeri, J.A., Tons, S.J. (1983) Photosynthetic adaptation of Solanum dulcamara L. to sun and shade environments. IV. A comparison of North American and European ecotypes. Oecologia 60, 348–352
Evans, J.R. (1983) Nitrogen and photosynthesis in the flag leaf of wheat (Triticum aestivum L.). Plant Physiol. 72, 297–302
Farquhar, G.D., von Caemmerer, S., Berry, J.A. (1980) A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species. Planta 149, 78–90
Ferrar, P.J., Osmond, C.B. (1986) Role of nitrogen nutrition in shade to sun acclimation by Solanum dulcamara. In: Kinetics of C3 photosynthesis, in press, Viil, J., Laisk, A., eds. Acad. Sci. Estonian SSR, Tallinn
Friedrich, J.W., Huffaker, R.C. (1980) Photosynthesis, leaf resistances and ribulose-1,5-bisphosphate carboxylase degradation in senescing barley leaves. Plant Physiol. 65, 1103–1107
Gauhl, E. (1976) Photosynthetic-response to varying light intensity in ecotypes of Solanum dulcamara L. from shaded and exposed habitats. Oecologia 22, 275–286
Gauhl, E. (1979) Sun and shade ecotypes of Solanum dulcamara L. Photosynthetic light dependence characteristics in relation to mild water stress. Oecologia 39, 61–70
Gibbs, A.J. (1983) Virus ecology — “struggle” of the genes. In: Encyclopedia of plant physiology, N.S., vol. 12C: Physiological plant ecology III, pp. 537–558. Lange, O.L., Nobel, P.S., Osmond, C.B., Zeigler, H., eds. Springer-Verlag, Berlin Heidelberg New York Tokyo
Makino, A., Mae, T., Ohira, K. (1984) Changes in the photosynthetic capacity in rice leaves from emergence through senescence. Analysis from ribulose-1,5-bisphosphate carboxylase and leaf conductance. Plant Cell Physiol. 25, 511–521
Matkin, D.A., Chandler, P.A. (1957) The UC-Type soil mixes. In: The U.C. system for producing healthy container-grown plants, Baker, K.F., ed. Univ. of Calif. Div. Agric. Sci., Agric, Exp. Stat. Extension Serv., Manual No. 23
Medina, E. (1971) Effect of nitrogen supply and light intensity during growth on the photosynthetic capacity and carboxy-dismutase activity of leaves of Atriplex patula ssp. Lastata. Carnegie Inst. Washington, Yearb. 70, 551–559
Osmond, C.B. (1983) Interactions between irradiance, nitrogen nutrition and water stress in the sun-shade-responses of Solanum dulcamara. Oecologia 57, 316–321
Osmond, B., Ferrar, P.J. (1984) Onset and recovery from photoinhibition in vivo: changes in leaf and chloroplast photosynthetic parameters. (Abstr.) Plant Physiol. 75, Suppl, 113
Powles, S.B. (1984) Inhibition of photosynthesis by high intensity visible light. Annu. Rev. Plant Physiol. 35, 15–44
Powles, S.B., Björkman, O. (1982) Photoinhibition of photosynthesis. Effect on chlorophyll fluorescence at 77 K in intact leaves and in chloroplast membranes of Nerium oleander. Planta 156, 97–107
Takano, Y., Tsunoda, S. (1971) Curvilinear regression of the leaf photosynthetic rate of leaf nitrogen content among strains of Orzyza species. Jap. J. Breed. 21, 69–76
Taylor, S.E., Terry, N. (1984) Factors limiting photosynthesis. V. Photochemical energy supply colimits photosynthesis at low values of intercellular CO2 concentration. Plant Physiol. 75, 82–86
von Caemmerer, S., Farquhar, G.D. (1981) Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta 153, 376–387
von Caemmerer, S., Farquhar, G.D. (1984) Effects of partial defoliation, changes of irradiance during growth, short term water stress and growth at enhanced p(CO2) on the photosynthetic capacity of leaves of Phaseolus vulgaris L. Planta 160, 320–329
Wong, S.C. (1979) Elevated atmospheric partial pressure of CO2 and plant growth. I. Interactions with nitrogen nutrition and photosynthetic capacity in C3 and C4 plants. Oecologia 44, 68–73
Wong, S.C., Cowan, I.R., Farquhar, G.D. (1985) Leaf conductance in relation to rate of CO2 assimilation. II. Influence of water stress and photoinhibition. Plant Physiol. 78, 830–834
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Ferrar, P.J., Osmond, C.B. Nitrogen supply as a factor influencing photoinhibition and photosynthetic acclimation after transfer of shade-grown Solanum dulcamara to bright light. Planta 168, 563–570 (1986). https://doi.org/10.1007/BF00392277
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DOI: https://doi.org/10.1007/BF00392277